IRF1

IRF1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1IF1
Identifiers
Aliases	IRF1, IRF-1, MAR, interferon regulatory factor 1
External IDs	OMIM: 147575; MGI: 96590; HomoloGene: 1658; GeneCards: IRF1; OMA:IRF1 - orthologs
Gene location (Mouse) Chr. Chromosome 11 (mouse)[1] Band 11 B1.3|11 32.0 cM Start 53,660,841 bp[1] End 53,669,200 bp[1]
RNA expression pattern Bgee Human Mouse (ortholog) n/a Top expressed in granulocyte jejunum mesenteric lymph nodes duodenum corneal stroma large intestine spleen colon ileum primary oocyte BioGPS More reference expression data
Gene ontology Molecular function DNA binding sequence-specific DNA binding DNA-binding transcription factor activity DNA-binding transcription activator activity, RNA polymerase II-specific RNA polymerase II cis-regulatory region sequence-specific DNA binding protein binding DNA-binding transcription factor activity, RNA polymerase II-specific Cellular component cytoplasm cytosol nucleoplasm nucleus Biological process apoptotic process regulation of CD8-positive, alpha-beta T cell proliferation CD8-positive, alpha-beta T cell differentiation regulation of innate immune response negative regulation of regulatory T cell differentiation regulation of transcription, DNA-templated cellular response to interferon-beta interferon-gamma-mediated signaling pathway immune system process regulation of adaptive immune response blood coagulation regulation of MyD88-dependent toll-like receptor signaling pathway transcription by RNA polymerase II transcription, DNA-templated regulation of cell cycle positive regulation of transcription, DNA-templated cellular response to mechanical stimulus defense response to virus type I interferon signaling pathway regulation of immune response regulation of gene expression innate immune response negative regulation of transcription, DNA-templated positive regulation of type I interferon production positive regulation of transcription by RNA polymerase II negative regulation of cell population proliferation positive regulation of interferon-beta production Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 3659 16362 Ensembl ENSG00000125347 ENSMUSG00000018899 UniProt P10914 P15314 RefSeq (mRNA) NM_002198 NM_001159393 NM_001159396 NM_008390 RefSeq (protein) NP_002189 NP_001341853 NP_001341854 NP_001152865 NP_001152868 NP_032416 Location (UCSC) n/a Chr 11: 53.66 – 53.67 Mb PubMed search [2] [3]
Wikidata
View/Edit Human View/Edit Mouse

Interferon regulatory factor 1 is a protein that in humans is encoded by the IRF1 gene.^[4][5]

Function

Interferon regulatory factor 1 was the first member of the interferon regulatory transcription factor (IRF) family identified. Initially described as a transcription factor able to activate expression of the cytokine Interferon beta,^[6] IRF-1 was subsequently shown to function as a transcriptional activator or repressor of a variety of target genes. IRF-1 regulates expression of target genes by binding to an interferon stimulated response element (ISRE) in their promoters. The IRF-1 protein binds to the ISRE via an N-terminal helix-turn-helix DNA binding domain,^[7] which is highly conserved among all IRF proteins.

Beyond its function as a transcription factor, IRF-1 has also been shown to trans-activate the tumour suppressor protein p53 through the recruitment of its co-factor p300.^[8]

IRF-1 has been shown to play roles in the immune response, regulating apoptosis, DNA damage and tumor suppression.^[9]

Regulation

It has been shown that the extreme C-terminus of IRF-1 regulates its ability to activate transcription, nanobodies targeting this domain (MF1) are able to increase IRF-1 activity.^[10]

Interactions

IRF1 has been shown to interact with:

• CHIP^[11]
• GAGE^[12]
• HSP70 / HSP90^[13]
• IRF8^[14][15]
• KPNA2^[16]
• MYD88^[17]
• PCAF^[18]
• STAT1^[19]
• TAT^[20]
• VEGFR2^[21]
• REDD2^[22]

See also

• IRF2
• Interferon regulatory factors

References

1. GRCm38: Ensembl release 89: ENSMUSG00000018899 – Ensembl, May 2017
2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. Maruyama M, Fujita T, Taniguchi T (Jun 1989). "Sequence of a cDNA coding for human IRF-1". Nucleic Acids Res. 17 (8): 3292. doi:10.1093/nar/17.8.3292. PMC 317732. PMID 2726461.
5. Itoh S, Harada H, Nakamura Y, White R, Taniguchi T (Nov 1991). "Assignment of the human interferon regulatory factor-1 (IRF1) gene to chromosome 5q23-q31". Genomics. 10 (4): 1097–9. doi:10.1016/0888-7543(91)90208-V. PMID 1680796.
6. Miyamoto M, Fujita T, Kimura Y, Maruyama M, Harada H, Sudo Y, et al. (September 1988). "Regulated expression of a gene encoding a nuclear factor, IRF-1, that specifically binds to IFN-beta gene regulatory elements". Cell. 54 (6): 903–13. doi:10.1016/S0092-8674(88)91307-4. PMID 3409321. S2CID 35063951.
7. Escalante CR, Yie J, Thanos D, Aggarwal AK (January 1998). "Structure of IRF-1 with bound DNA reveals determinants of interferon regulation". Nature. 391 (6662): 103–6. Bibcode:1998Natur.391..103E. doi:10.1038/34224. PMID 9422515. S2CID 4394514.
8. Dornan D, Eckert M, Wallace M, Shimizu H, Ramsay E, Hupp TR, et al. (November 2004). "Interferon regulatory factor 1 binding to p300 stimulates DNA-dependent acetylation of p53". Mol. Cell. Biol. 24 (22): 10083–98. doi:10.1128/MCB.24.22.10083-10098.2004. PMC 525491. PMID 15509808.
9. "Entrez Gene: IRF1 interferon regulatory factor 1".
10. Möller A, Pion E, Narayan V, Ball KL (December 2010). "Intracellular activation of interferon regulatory factor-1 by nanobodies to the multifunctional (Mf1) domain". J. Biol. Chem. 285 (49): 38348–61. doi:10.1074/jbc.M110.149476. PMC 2992268. PMID 20817723.
11. Narayan V, Pion E, Landré V, Müller P, Ball KL (October 2010). "Docking dependent ubiquitination of the interferon regulatory factor-1 tumour suppressor protein by the ubiquitin ligase CHIP". J Biol Chem. 286 (1): 607–19. doi:10.1074/jbc.M110.153122. PMC 3013021. PMID 20947504.
12. Kular RK, Yehiely F, Kotlo KU, Cilensek ZM, Bedi R, Deiss LP (October 2009). "GAGE, an antiapoptotic protein binds and modulates the expression of nucleophosmin/B23 and interferon regulatory factor 1". J. Interferon Cytokine Res. 29 (10): 645–55. doi:10.1089/jir.2008.0099. PMID 19642896.
13. Narayan V, Eckert M, Zylicz A, Zylicz M, Ball KL (September 2009). "Cooperative regulation of the interferon regulatory factor-1 tumor suppressor protein by core components of the molecular chaperone machinery". J Biol Chem. 284 (38): 25889–99. doi:10.1074/jbc.M109.019505. PMC 2757990. PMID 19502235.
14. Schaper F, Kirchhoff S, Posern G, Köster M, Oumard A, Sharf R, et al. (October 1998). "Functional domains of interferon regulatory factor I (IRF-1)". Biochem. J. 335 (1): 147–57. doi:10.1042/bj3350147. PMC 1219763. PMID 9742224.
15. Sharf R, Azriel A, Lejbkowicz F, Winograd SS, Ehrlich R, Levi BZ (June 1995). "Functional domain analysis of interferon consensus sequence binding protein (ICSBP) and its association with interferon regulatory factors". J. Biol. Chem. 270 (22): 13063–9. doi:10.1074/jbc.270.22.13063. PMID 7768900.
16. Umegaki N, Tamai K, Nakano H, Moritsugu R, Yamazaki T, Hanada K, et al. (June 2007). "Differential regulation of karyopherin alpha 2 expression by TGF-beta1 and IFN-gamma in normal human epidermal keratinocytes: evident contribution of KPNA2 for nuclear translocation of IRF-1". J. Invest. Dermatol. 127 (6): 1456–64. doi:10.1038/sj.jid.5700716. PMID 17255955.
17. Negishi H, Fujita Y, Yanai H, Sakaguchi S, Ouyang X, Shinohara M, et al. (October 2006). "Evidence for licensing of IFN-gamma-induced IFN regulatory factor 1 transcription factor by MyD88 in Toll-like receptor-dependent gene induction program". Proc. Natl. Acad. Sci. U.S.A. 103 (41): 15136–41. Bibcode:2006PNAS..10315136N. doi:10.1073/pnas.0607181103. PMC 1586247. PMID 17018642.
18. Masumi A, Wang IM, Lefebvre B, Yang XJ, Nakatani Y, Ozato K (March 1999). "The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness". Mol. Cell. Biol. 19 (3): 1810–20. doi:10.1128/MCB.19.3.1810. PMC 83974. PMID 10022868.
19. Chatterjee-Kishore M, van Den Akker F, Stark GR (July 2000). "Adenovirus E1A down-regulates LMP2 transcription by interfering with the binding of stat1 to IRF1". J. Biol. Chem. 275 (27): 20406–11. doi:10.1074/jbc.M001861200. PMID 10764778.
20. Sgarbanti M, Borsetti A, Moscufo N, Bellocchi MC, Ridolfi B, Nappi F, et al. (May 2002). "Modulation of human immunodeficiency virus 1 replication by interferon regulatory factors". J. Exp. Med. 195 (10): 1359–70. doi:10.1084/jem.20010753. PMC 2193759. PMID 12021315.
21. Lee JH, Chun T, Park SY, Rho SB (September 2008). "Interferon regulatory factor-1 (IRF-1) regulates VEGF-induced angiogenesis in HUVECs". Biochim. Biophys. Acta. 1783 (9): 1654–62. doi:10.1016/j.bbamcr.2008.04.006. PMID 18472010.
22. Gupta M, Rath PC (April 2014). "Interferon regulatory factor-1 (IRF-1) interacts with regulated in development and DNA damage response 2 (REDD2) in the cytoplasm of mouse bone marrow cells". International Journal of Biological Macromolecules. 65: 41–50. doi:10.1016/j.ijbiomac.2014.01.005. PMID 24412152.

Further reading

• Harada H, Taniguchi T, Tanaka N (1999). "The role of interferon regulatory factors in the interferon system and cell growth control". Biochimie. 80 (8–9): 641–50. doi:10.1016/S0300-9084(99)80017-0. PMID 9865486.
• Pitha PM, Au WC, Lowther W, Juang YT, Schafer SL, Burysek L, et al. (1999). "Role of the interferon regulatory factors (IRFs) in virus-mediated signaling and regulation of cell growth". Biochimie. 80 (8–9): 651–8. doi:10.1016/S0300-9084(99)80018-2. PMID 9865487.
• Yu-Lee L (2002). "Stimulation of interferon regulatory factor-1 by prolactin". Lupus. 10 (10): 691–9. doi:10.1191/096120301717164921. PMID 11721695. S2CID 13547888.
• Pine R (2002). "IRF and tuberculosis". J. Interferon Cytokine Res. 22 (1): 15–25. doi:10.1089/107999002753452629. PMID 11846972.
• Romeo G, Fiorucci G, Chiantore MV, Percario ZA, Vannucchi S, Affabris E (2002). "IRF-1 as a negative regulator of cell proliferation". J. Interferon Cytokine Res. 22 (1): 39–47. doi:10.1089/107999002753452647. PMID 11846974.
• Cha Y, Sims SH, Romine MF, Kaufmann M, Deisseroth AB (1992). "Human interferon regulatory factor 1: intron-exon organization". DNA Cell Biol. 11 (8): 605–11. doi:10.1089/dna.1992.11.605. PMID 1382447.
• Harada H, Fujita T, Miyamoto M, Kimura Y, Maruyama M, Furia A, et al. (1989). "Structurally similar but functionally distinct factors, IRF-1 and IRF-2, bind to the same regulatory elements of IFN and IFN-inducible genes". Cell. 58 (4): 729–39. doi:10.1016/0092-8674(89)90107-4. PMID 2475256. S2CID 2033941.
• Miyamoto M, Fujita T, Kimura Y, Maruyama M, Harada H, Sudo Y, et al. (1988). "Regulated expression of a gene encoding a nuclear factor, IRF-1, that specifically binds to IFN-beta gene regulatory elements". Cell. 54 (6): 903–13. doi:10.1016/S0092-8674(88)91307-4. PMID 3409321. S2CID 35063951.
• Harada H, Takahashi E, Itoh S, Harada K, Hori TA, Taniguchi T (1994). "Structure and regulation of the human interferon regulatory factor 1 (IRF-1) and IRF-2 genes: implications for a gene network in the interferon system". Mol. Cell. Biol. 14 (2): 1500–9. doi:10.1128/MCB.14.2.1500. PMC 358505. PMID 7507207.
• Sharf R, Azriel A, Lejbkowicz F, Winograd SS, Ehrlich R, Levi BZ (1995). "Functional domain analysis of interferon consensus sequence binding protein (ICSBP) and its association with interferon regulatory factors". J. Biol. Chem. 270 (22): 13063–9. doi:10.1074/jbc.270.22.13063. PMID 7768900.
• Willman CL, Sever CE, Pallavicini MG, Harada H, Tanaka N, Slovak ML, et al. (1993). "Deletion of IRF-1, mapping to chromosome 5q31.1, in human leukemia and preleukemic myelodysplasia". Science. 259 (5097): 968–71. Bibcode:1993Sci...259..968W. doi:10.1126/science.8438156. PMID 8438156. S2CID 40142541.
• Drew PD, Franzoso G, Becker KG, Bours V, Carlson LM, Siebenlist U, et al. (1997). "NF kappa B and interferon regulatory factor 1 physically interact and synergistically induce major histocompatibility class I gene expression". J. Interferon Cytokine Res. 15 (12): 1037–45. doi:10.1089/jir.1995.15.1037. PMID 8746784.
• Ronco LV, Karpova AY, Vidal M, Howley PM (1998). "Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity". Genes Dev. 12 (13): 2061–72. doi:10.1101/gad.12.13.2061. PMC 316980. PMID 9649509.
• Nozawa H, Oda E, Ueda S, Tamura G, Maesawa C, Muto T, et al. (1998). "Functionally inactivating point mutation in the tumor-suppressor IRF-1 gene identified in human gastric cancer". Int. J. Cancer. 77 (4): 522–7. doi:10.1002/(SICI)1097-0215(19980812)77:4<522::AID-IJC8>3.0.CO;2-W. PMID 9679752. S2CID 25951704.
• Schaper F, Kirchhoff S, Posern G, Köster M, Oumard A, Sharf R, et al. (1998). "Functional domains of interferon regulatory factor I (IRF-1)". Biochem. J. 335 (1): 147–57. doi:10.1042/bj3350147. PMC 1219763. PMID 9742224.
• Masumi A, Wang IM, Lefebvre B, Yang XJ, Nakatani Y, Ozato K (1999). "The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness". Mol. Cell. Biol. 19 (3): 1810–20. doi:10.1128/MCB.19.3.1810. PMC 83974. PMID 10022868.

External links

• IRF1+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
• FactorBook IRF1

This article incorporates text from the United States National Library of Medicine, which is in the public domain.