ZNF366

ZNF366
Identifiers
Aliases	ZNF366, DCSCRIPT, zinc finger protein 366, DC-SCRIPT
External IDs	OMIM: 610159; MGI: 2178429; HomoloGene: 17637; GeneCards: ZNF366; OMA:ZNF366 - orthologs
Gene location (Human) Chr. Chromosome 5 (human)[1] Band 5q13.2|5q13.2 Start 72,439,903 bp[1] End 72,507,410 bp[1]
Gene location (Mouse) Chr. Chromosome 13 (mouse)[2] Band 13|13 D1 Start 99,321,331 bp[2] End 99,387,164 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sural nerve testicle epithelium of colon smooth muscle tissue gonad apex of heart appendix left ventricle lymph node subcutaneous adipose tissue Top expressed in lung muscle of thigh quadriceps femoris muscle muscle tissue spleen skeletal muscle tissue white adipose tissue adrenal gland Cerebellum human kidney More reference expression data BioGPS n/a
Gene ontology Molecular function DNA binding estrogen receptor binding protein binding transcription corepressor activity metal ion binding nucleic acid binding DNA-binding transcription factor activity, RNA polymerase II-specific Cellular component nucleus Biological process negative regulation of transcription by RNA polymerase II negative regulation of intracellular estrogen receptor signaling pathway regulation of transcription, DNA-templated transcription, DNA-templated response to estrogen Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 167465 238803 Ensembl ENSG00000178175 ENSMUSG00000050919 UniProt Q8N895 Q6NS86 RefSeq (mRNA) NM_152625 NM_001004149 RefSeq (protein) NP_689838 NP_001004149 Location (UCSC) Chr 5: 72.44 – 72.51 Mb Chr 13: 99.32 – 99.39 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Zinc finger protein 366, also known as DC-SCRIPT (Dendritic cell-specific transcript), is a protein that in humans is encoded by the ZNF366 gene.^[5] The ZNF366 gene was first identified in a DNA comparison study between 85 kb of Fugu rubripes sequence containing 17 genes with its homologous loci in the human draft genome.^[6]

Function

In 2006, DC-SCRIPT was isolated and characterized in human monocyte-derived dendritic cells (mo-DCs).^[7]

DC-SCRIPT contains a DNA-binding domain (11 C₂H₂ zinc (Zn) fingers), flanked by a proline-rich and an acidic region, which can interact with C-terminal-binding protein 1 (CtBP1), a global corepressor. In the immune system of both mice and humans, DC-SCRIPT was found to be specifically expressed in dendritic cells (DCs).^[8]

In COS-1 cells, DC-SCRIPT was shown to interact with the estrogen receptor DNA-binding domain (ERDBD) and represses ER activity through the association with RIP140, CtBP and histone deacetylases.^[9]

In DCs, DC-SCRIPT was found to be highly expressed in type one conventional DCs (cDC1s) under the control of PU.1.^[10] The presence of DC-SCRIPT is important for the cDC1s lineage specification via maintaining Interferon regulatory factor 8 (IRF8) expression. The DC-SCRIPT deficient cDC1s had impaired capacity to capture and present cell-associated antigens and to secrete IL-12p40.^[11]

Breast cancer

In 2010, it was shown that DC-SCRIPT can act as a coregulator of multiple nuclear receptors having opposite effects on type I vs type II NRs. DC-SCRIPT is able to repress ER and PR mediated transcription, whereas it can activate transcription mediated by RAR and PPAR. In the same study, it was shown that breast tumor tissue expresses lower levels of DC-SCRIPT than normal breast tissue from the same patient and that DC-SCRIPT mRNA expression is an independent prognostic factor for good survival of breast cancer patients with estrogen receptor- and/or progesterone receptor-positive tumors.^[12]

References

1. GRCh38: Ensembl release 89: ENSG00000178175 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000050919 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: ZNF366 zinc finger protein 366".
6. Gilligan P, Brenner S, Venkatesh B (July 2002). "Fugu and human sequence comparison identifies novel human genes and conserved non-coding sequences". Gene. 294 (1–2): 35–44. doi:10.1016/S0378-1119(02)00793-X. PMID 12234665.
7. Triantis V, Trancikova DE, Looman MW, Hartgers FC, Janssen RA, Adema GJ (January 2006). "Identification and characterization of DC-SCRIPT, a novel dendritic cell-expressed member of the zinc finger family of transcriptional regulators". Journal of Immunology. 176 (2): 1081–9. doi:10.4049/jimmunol.176.2.1081. PMID 16393996.
8. Triantis V, Moulin V, Looman MW, Hartgers FC, Janssen RA, Adema GJ (May 2006). "Molecular characterization of the murine homologue of the DC-derived protein DC-SCRIPT". Journal of Leukocyte Biology. 79 (5): 1083–91. doi:10.1189/jlb.1005588. hdl:2066/49437. PMID 16522745. S2CID 27847791.
9. Lopez-Garcia J, Periyasamy M, Thomas RS, Christian M, Leao M, Jat P, et al. (2006). "ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases". Nucleic Acids Research. 34 (21): 6126–36. doi:10.1093/nar/gkl875. PMC 1693901. PMID 17085477.
10. Chopin M, Lun AT, Zhan Y, Schreuder J, Coughlan H, D'Amico A, et al. (January 2019). "Transcription Factor PU.1 Promotes Conventional Dendritic Cell Identity and Function via Induction of Transcriptional Regulator DC-SCRIPT". Immunity. 50 (1): 77–90.e5. doi:10.1016/j.immuni.2018.11.010. PMID 30611612.
11. Zhang S, Coughlan HD, Ashayeripanah M, Seizova S, Kueh AJ, Brown DV, et al. (April 2021). "Type 1 conventional dendritic cell fate and function are controlled by DC-SCRIPT". Science Immunology. 6 (58): eabf4432. doi:10.1126/sciimmunol.abf4432. PMID 33811060. S2CID 232771588.
12. Ansems M, Hontelez S, Looman MW, Karthaus N, Bult P, Bonenkamp JJ, et al. (January 2010). "DC-SCRIPT: nuclear receptor modulation and prognostic significance in primary breast cancer". Journal of the National Cancer Institute. 102 (1): 54–68. doi:10.1093/jnci/djp441. PMID 20008677.

Further reading

• Hartley JL, Temple GF, Brasch MA (November 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, et al. (March 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, et al. (October 2004). "From ORFeome to biology: a functional genomics pipeline". Genome Research. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, et al. (January 2006). "The LIFEdb database in 2006". Nucleic Acids Research. 34 (Database issue): D415-8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.

External links

• ZNF366+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.