Twelve teams from Division 1 2010 or lower, two of the three teams which earned promotion to 2010 Superettan (not IK Brage) and the bottom eight teams from 2009 Superettan entered in this round. They were joined by the 26 preliminary round winners.
Two demoted teams from 2009 Allsvenskan and six teams ranked 3rd through 8th in 2009 Superettan entered in this round, joining 24 winners from Round 1.