The 2009–10 Toto Cup Al was the twenty-eighth season of the third most important football tournament in Israel since its introduction and sixth under the current format. It was held in two stages. First, sixteen Premier League teams were divided into four groups. The winners and runners-up, were advanced to the knockout phase. Quarter-finals, Semi-finals and Final was held as one-legged matches, with the Final played at the Ramat Gan Stadium.
The defending champions were Maccabi Tel Aviv, making it their third Toto Cup title overall.
On 26 January 2010, Beitar Jerusalem won the 2009–10 Toto Cup Al making it their second Toto Cup title overall.
Group stage
The matches were played from August 1 to December 15, 2009.
^ abDue to the Court's decision, no points were awarded for the game between Maccabi Ahi Nazareth and Hapoel Haifa, after Hapoel Haifa fielded an ineligible player, The original game was finished in a 2–0 win for Hapoel Haifa.
^Due to the Court's decision, no points were awarded for the game between Maccabi Ahi Nazareth and Hapoel Haifa, after Hapoel Haifa fielded an ineligible player, The original game was finished in a 2–0 win for Hapoel Haifa.
^ abMaccabi Netanya received a technical loss of 0–3 to Beitar Jerusalem for fielding an ineligible player, The original game was finished in a 3–3 draw.
^Maccabi Netanya received a technical loss of 0–3 to Beitar Jerusalem for fielding an ineligible player, The original game was finished in a 3–3 draw.