DnaC

DNA replication gene dnaC
Identifiers
Organism	Escherichia coli (str. K-12 substr. MG1655)
Symbol	dnaC
Entrez	948864
RefSeq (Prot)	NP_418781.1
UniProt	P0AEF0
Other data
EC number	2.7.7.7
Chromosome	genome: 4.6 - 4.6 Mb
Search for Structures Swiss-model Domains InterPro

dnaC is a loading factor that complexes with the C-terminus of helicase dnaB and inhibits it from unwinding the dsDNA at a replication fork.^[1] A dnaB and dnaC associate near the dnaA bound origin for each of the ssDNA.^[1] One dnaB-dnaC complex is oriented in the opposite direction to the other dnaB-dnaC complex due to the antiparallel nature of DNA. Because they are oriented in opposite directions, one dnaB-dnaC complex will complex with dnaA from the N-terminus of dnaB whereas the other dnaB-dnaC complex will complex with dnaA from the dnaC. After the assembly of dnaG onto the N-terminus of dnaB, dnaC is released and dnaB will be allowed to begin unwinding dsDNA to make room for DNA polymerase III to begin synthesizing the daughter strands.^[1]

This interaction of dnaC with dnaB requires the hydrolysis of ATP.^[2]

References

1. Slonczewski JL, Foster JW, Gillen KM (2009). Microbiology: an evolving science (1st ed.). New York: W.W. Norton & Co. ISBN 978-0-393-97857-5.
2. Kobori JA, Kornberg A (November 1982). "The Escherichia coli dnaC gene product. III. Properties of the dnaB-dnaC protein complex". The Journal of Biological Chemistry. 257 (22): 13770–5. PMID 6292205. Archived from the original on 2007-09-29. Retrieved 2007-08-20.

External links

• DnaC+protein,+E+coli at the U.S. National Library of Medicine Medical Subject Headings (MeSH)