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ORC1
Identifiers
Aliases	ORC1, HSORC1L, PARC1, origin recognition complex subunit 1
External IDs	OMIM: 601902; MGI: 1328337; HomoloGene: 31221; GeneCards: ORC1; OMA:ORC1 - orthologs
Gene location (Human)
Chr.	Chromosome 1 (human)
End	52,404,423 bp
Gene location (Mouse)
Chr.	Chromosome 4 (mouse)
End	108,472,030 bp
RNA expression pattern
	Top expressed in
	testicle; ; ventricular zone; ; gonad; ; ganglionic eminence; ; bone marrow; ; mucosa of transverse colon; ; secondary oocyte; ; rectum; ; stromal cell of endometrium; ; bone marrow cells;
	Top expressed in
	zygote; ; secondary oocyte; ; primary oocyte; ; epiblast; ; tail of embryo; ; genital tubercle; ; primitive streak; ; embryo; ; embryo; ; blastocyst;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	DNA binding; nucleotide binding; chromatin binding; protein binding; ATP binding; DNA replication origin binding; metal ion binding;
Cellular component	nuclear origin of replication recognition complex; plasma membrane; nucleolus; origin recognition complex; nucleus; nucleoplasm; cytosol;
Biological process	G1 phase; DNA replication; regulation of transcription involved in G1/S transition of mitotic cell cycle; DNA replication initiation; G1/S transition of mitotic cell cycle; mitotic cell cycle; mitotic DNA replication checkpoint signaling;
	Sources:Amigo / QuickGO
Orthologs
	4998
	18392
	ENSG00000085840
	ENSMUSG00000028587
	Q13415
	Q9Z1N2
	NM_001190818; NM_001190819; NM_004153
	NM_001014425; NM_011015
	NP_001177747; NP_001177748; NP_004144
	NP_035145
	Wikidata
View/Edit Human	View/Edit Mouse

Origin recognition complex subunit 1 is a protein that in humans is encoded by the ORC1 gene.^[5]^[6] It is closely related to CDC6, and both are the same protein in archaea.^[7]

Function

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of the pre-replication complex, which includes additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the origin recognition complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MYST2/HBO1), a protein involved in control of transcription silencing.^[6]

Interactions

ORC1 has been shown to interact with:

CDC45-related protein^[8] and
CDC6,^[8]^[9]
Cell division cycle 7-related protein kinase,^[8]
Cyclin-dependent kinase 2,^[10]
MCM2,^[8]
MCM4,^[8]
MCM6,^[8]
MCM7,^[8]
MYST2,^[11]
ORC2,^[8]^[12]^[13]^[10]
ORC4,^[8]
ORC5,^[8] and
SKP2.^[10]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000085840 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000028587 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Eki T, Okumura K, Amin A, Ishiai M, Abe M, Nogami M, Taguchi H, Hurwitz J, Murakami Y, Hanaoka F (Sep 1996). "Mapping of the human homologue (ORC1L) of the yeast origin recognition complex subunit 1 gene to chromosome band 1p32". Genomics. 36 (3): 559–61. doi:10.1006/geno.1996.0511. PMID 8884289.
^ ^a ^b "Entrez Gene: ORC1L origin recognition complex, subunit 1-like (yeast)".
^ Ausiannikava, Darya; Allers, Thorsten (31 January 2017). "Diversity of DNA Replication in the Archaea". Genes. 8 (2): 56. doi:10.3390/genes8020056. PMC 5333045. PMID 28146124.
^ ^a ^b ^c ^d ^e ^f ^g ^h ⁱ ^j Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". Journal of Molecular Biology. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612.
^ Saha P, Chen J, Thome KC, Lawlis SJ, Hou ZH, Hendricks M, Parvin JD, Dutta A (May 1998). "Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase". Molecular and Cellular Biology. 18 (5): 2758–67. doi:10.1128/mcb.18.5.2758. PMC 110655. PMID 9566895.
^ ^a ^b ^c Méndez J, Zou-Yang XH, Kim SY, Hidaka M, Tansey WP, Stillman B (Mar 2002). "Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication". Molecular Cell. 9 (3): 481–91. doi:10.1016/s1097-2765(02)00467-7. PMID 11931757.
^ Iizuka M, Stillman B (Aug 1999). "Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein". The Journal of Biological Chemistry. 274 (33): 23027–34. doi:10.1074/jbc.274.33.23027. PMID 10438470.
^ Fujita M, Ishimi Y, Nakamura H, Kiyono T, Tsurumi T (Mar 2002). "Nuclear organization of DNA replication initiation proteins in mammalian cells". The Journal of Biological Chemistry. 277 (12): 10354–61. doi:10.1074/jbc.M111398200. PMID 11779870.
^ Vashee S, Simancek P, Challberg MD, Kelly TJ (Jul 2001). "Assembly of the human origin recognition complex". The Journal of Biological Chemistry. 276 (28): 26666–73. doi:10.1074/jbc.M102493200. PMID 11323433.

External links

Overview of all the structural information available in the PDB for UniProt: Q13415 (Human Origin recognition complex subunit 1) at the PDBe-KB.
Overview of all the structural information available in the PDB for UniProt: Q9Z1N2 (Mouse Origin recognition complex subunit 1) at the PDBe-KB.