MCM3

MCM3
Identifiers
Aliases	MCM3, HCC5, P1-P1.h, RLFB, minichromosome maintenance complex component 3
External IDs	OMIM: 602693; MGI: 101845; HomoloGene: 1791; GeneCards: MCM3; OMA:MCM3 - orthologs
Gene location (Human) Chr. Chromosome 6 (human)[1] Band 6p12.2 Start 52,264,009 bp[1] End 52,284,881 bp[1]
Gene location (Mouse) Chr. Chromosome 1 (mouse)[2] Band 1|1 A4 Start 20,873,192 bp[2] End 20,890,536 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ventricular zone embryo ganglionic eminence mucosa of transverse colon appendix rectum lymph node gonad trabecular bone body of uterus Top expressed in somite epiblast mandibular prominence fetal liver hematopoietic progenitor cell maxillary prominence ventricular zone primitive streak tail of embryo otic placode abdominal wall More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding nucleotide binding helicase activity protein binding hydrolase activity ATP binding DNA replication origin binding single-stranded DNA binding Cellular component cytoplasm centrosome membrane nucleoplasm perinuclear region of cytoplasm alpha DNA polymerase:primase complex MCM complex nucleus nucleolus intracellular membrane-bounded organelle Biological process G1 phase DNA replication DNA replication initiation cell cycle G1/S transition of mitotic cell cycle double-strand break repair via break-induced replication pre-replicative complex assembly involved in nuclear cell cycle DNA replication DNA strand elongation involved in DNA replication mitotic DNA replication initiation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4172 17215 Ensembl ENSG00000112118 ENSMUSG00000041859 UniProt P25205 Q7Z6P5 P25206 RefSeq (mRNA) NM_002388 NM_001270472 NM_008563 RefSeq (protein) NP_001257401 NP_002379 NP_001353298 NP_001353299 NP_001353300 NP_001353301 NP_001353302 NP_001353303 NP_001353304 NP_032589 Location (UCSC) Chr 6: 52.26 – 52.28 Mb Chr 1: 20.87 – 20.89 Mb PubMed search [3] [4]
Wikidata
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DNA replication licensing factor MCM3 is a protein that in humans is encoded by the MCM3 gene.^[5]

Function

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.^[6]

Interactions

MCM3 has been shown to interact with:

• CDC45-related protein,^[7]
• CDC6,^[8][9]
• DBF4,^[7]
• MCM2^[7]
• MCM3AP,^[10][11][12]
• MCM5,^[7][13]
• MCM7,^{[7][8][14][15]}
• ORC4L,^[7] and
• ORC5L.^[7]

See also

• Mini Chromosome Maintenance

References

1. GRCh38: Ensembl release 89: ENSG00000112118 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000041859 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Thömmes P, Fett R, Schray B, Burkhart R, Barnes M, Kennedy C, Brown NC, Knippers R (April 1992). "Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein". Nucleic Acids Res. 20 (5): 1069–74. doi:10.1093/nar/20.5.1069. PMC 312092. PMID 1549468.
6. "Entrez Gene: MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)".
7. Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612.
8. Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M, Tsurumi T (September 1999). "Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation". J. Biol. Chem. 274 (36): 25927–32. doi:10.1074/jbc.274.36.25927. PMID 10464337.
9. Méndez J, Stillman B (November 2000). "Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis". Mol. Cell. Biol. 20 (22): 8602–12. doi:10.1128/mcb.20.22.8602-8612.2000. PMC 102165. PMID 11046155.
10. Takei Y, Assenberg M, Tsujimoto G, Laskey R (November 2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi:10.1074/jbc.C200442200. PMID 12226073.
11. Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (April 2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood. 95 (7): 2321–8. doi:10.1182/blood.V95.7.2321. PMID 10733502.
12. Takei Y, Tsujimoto G (August 1998). "Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization". J. Biol. Chem. 273 (35): 22177–80. doi:10.1074/jbc.273.35.22177. PMID 9712829.
13. DaFonseca CJ, Shu F, Zhang JJ (March 2001). "Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma". Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3034–9. Bibcode:2001PNAS...98.3034D. doi:10.1073/pnas.061487598. PMC 30602. PMID 11248027.
14. Fujita M, Kiyono T, Hayashi Y, Ishibashi M (April 1997). "In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP". J. Biol. Chem. 272 (16): 10928–35. doi:10.1074/jbc.272.16.10928. PMID 9099751.
15. Fujita M, Ishimi Y, Nakamura H, Kiyono T, Tsurumi T (March 2002). "Nuclear organization of DNA replication initiation proteins in mammalian cells". J. Biol. Chem. 277 (12): 10354–61. doi:10.1074/jbc.M111398200. PMID 11779870.

Further reading

• Musahl C, Schulte D, Burkhart R, Knippers R (1995). "A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins". Eur. J. Biochem. 230 (3): 1096–101. doi:10.1111/j.1432-1033.1995.tb20660.x. PMID 7601140.
• Schulte D, Burkhart R, Musahl C, Hu B, Schlatterer C, Hameister H, Knippers R (1995). "Expression, phosphorylation and nuclear localization of the human P1 protein, a homologue of the yeast Mcm 3 replication protein". J. Cell Sci. 108 (4): 1381–9. doi:10.1242/jcs.108.4.1381. PMID 7615659.
• Starborg M, Brundell E, Gell K, Larsson C, White I, Daneholt B, Höög C (1995). "A murine replication protein accumulates temporarily in the heterochromatic regions of nuclei prior to initiation of DNA replication". J. Cell Sci. 108 (3): 927–34. doi:10.1242/jcs.108.3.927. PMID 7622621.
• Burkhart R, Schulte D, Hu D, Musahl C, Göhring F, Knippers R (1995). "Interactions of human nuclear proteins P1Mcm3 and P1Cdc46". Eur. J. Biochem. 228 (2): 431–8. doi:10.1111/j.1432-1033.1995.tb20281.x. PMID 7705359.
• Kubota Y, Mimura S, Nishimoto S, Takisawa H, Nojima H (1995). "Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor". Cell. 81 (4): 601–9. doi:10.1016/0092-8674(95)90081-0. PMID 7758114. S2CID 18797719.
• Madine MA, Khoo CY, Mills AD, Laskey RA (1995). "MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells". Nature. 375 (6530): 421–4. Bibcode:1995Natur.375..421M. doi:10.1038/375421a0. PMID 7760938. S2CID 4316044.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Hu B, Burkhart R, Schulte D, Musahl C, Knippers R (1994). "The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins". Nucleic Acids Res. 21 (23): 5289–93. doi:10.1093/nar/21.23.5289-a. PMC 310560. PMID 8265339.
• Schulte D, Richter A, Burkhart R, Musahl C, Knippers R (1996). "Properties of the human nuclear protein p85Mcm. Expression, nuclear localization and interaction with other Mcm proteins". Eur. J. Biochem. 235 (1–2): 144–51. doi:10.1111/j.1432-1033.1996.00144.x. PMID 8631321.
• Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H (1996). "Binding of human minichromosome maintenance proteins with histone H3". J. Biol. Chem. 271 (39): 24115–22. doi:10.1074/jbc.271.39.24115. PMID 8798650.
• Sato N, Arai K, Masai H (1997). "Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7". EMBO J. 16 (14): 4340–51. doi:10.1093/emboj/16.14.4340. PMC 1170060. PMID 9250678.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Takei Y, Tsujimoto G (1998). "Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization". J. Biol. Chem. 273 (35): 22177–80. doi:10.1074/jbc.273.35.22177. PMID 9712829.
• Zhang JJ, Zhao Y, Chait BT, Lathem WW, Ritzi M, Knippers R, Darnell JE (1999). "Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation". EMBO J. 17 (23): 6963–71. doi:10.1093/emboj/17.23.6963. PMC 1171044. PMID 9843502.
• Yankulov K, Todorov I, Romanowski P, Licatalosi D, Cilli K, McCracken S, Laskey R, Bentley DL (1999). "MCM Proteins Are Associated with RNA Polymerase II Holoenzyme". Mol. Cell. Biol. 19 (9): 6154–63. doi:10.1128/MCB.19.9.6154. PMC 84545. PMID 10454562.
• Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M, Tsurumi T (1999). "Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation". J. Biol. Chem. 274 (36): 25927–32. doi:10.1074/jbc.274.36.25927. PMID 10464337.
• Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood. 95 (7): 2321–8. doi:10.1182/blood.V95.7.2321. PMID 10733502.
• Hofmann Y, Becker J, Wright F, Avner ED, Mrug M, Guay-Woodford LM, Somlo S, Zerres K, Germino GG, Onuchic LF (2000). "Genomic structure of the gene for the human P1 protein (MCM3) and its exclusion as a candidate for autosomal recessive polycystic kidney disease". Eur. J. Hum. Genet. 8 (3): 163–6. doi:10.1038/sj.ejhg.5200426. PMID 10780780.
• Abe E, Kuwahara K, Yoshida M, Suzuki M, Terasaki H, Matsuo Y, Takahashi EI, Sakaguchi N (2000). "Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication". Gene. 255 (2): 219–27. doi:10.1016/S0378-1119(00)00336-X. PMID 11024281.