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Minichromosome maintenance complex component 4
Identifikatori
Simboli	MCM4; CDC21; CDC54; NKCD; NKGCD; P1-CDC21; hCdc21
Vanjski ID	OMIM: 602638 MGI: 103199 HomoloGene: 40496 GeneCards: MCM4 Gene
EC broj	3.6.4.12
Ontologija gena
Molekularna funkcija	• vezivanje jednolančane DNK; • aktivnost ATP-zavisne DNK helikaze; • proteinsko vezivanje;
Celularna komponenta	• nukleus; • nukleoplazma; • jedarce;
Biološki proces	• G1/S tranzicija mitotičkog ćelijskog ciklusa; • mitotički ćelijski ciklus; • DNK replikacija;
Pregled RNK izražavanja
	podaci
Ortolozi

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Faktor licenciranja DNK replikacije MCM4 je protein koji je kod ljudi kodiran MCM4 genom.^[1]

Protein kodiran ovim genom je jedan od visoko konzerviranjih proteina održavanja mini-hromozoma (MCM) koji učestvuju u inicijaciji replikacije eukariotskog genoma. Heksamerni proteinski kompleks formiran od MCM proteina je ključna komponenta prereplikacionog kompleksa (pre-RC). On učestuje u formiranju replikacionih viljuški i regrutaciji drugih srodnih proteina replikacije DNK. Ovaj protein formira kompleks sa MCM2, 6, i 7, i pokazano je da reguliše helikaznu aktivnost kompleksa. Smatra se da deluje kao enzim odvijanja DNK. Fosforilacija ovog proteina posredstvom CDC2 kinaze redukuje DNK helikaznu aktivnost i hromatinsko vezivanje MCM kompleksa. Ovaj gen je mapiran na region hromozoma 8 pored PRKDC/DNA-PK, DNK-aktivirane proteinske kinaze koja učestvuje u popravci prekida dvolančane DNK. Alternativno splajsovane transkriptne varijante kodiraju isti protein.^[2]

Povezano

Održavanje minihromozoma

Interakcije

MCM4 formira interakcije sa ORC1L,^[3] ORC2L,^[3] replikacionim proteinom A1,^[3] ORC4L,^[3] ORC5L,^[3] ORC3L,^[3] MCM6,^[3]^[4]^[5]^[6] MCM7,^[4]^[5]^[6]^[7] MCM2,^[3]^[4]^[5]^[8] CDC7^[3] and ORC6L.^[3]

Reference

↑ Musahl C, Schulte D, Burkhart R, Knippers R (August 1995). „A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins”. Eur J Biochem 230 (3): 1096–101. DOI:10.1111/j.1432-1033.1995.tb20660.x. PMID 7601140.
↑ „Entrez Gene: MCM4 MCM4 minichromosome maintenance deficient 4 (S. cerevisiae)”.
↑ ^3,00 ^3,01 ^3,02 ^3,03 ^3,04 ^3,05 ^3,06 ^3,07 ^3,08 ^3,09 Kneissl, Margot; Pütter Vera; Szalay Aladar A; Grummt Friedrich (March 2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. (England) 327 (1): 111–28. DOI:10.1016/S0022-2836(03)00079-2. ISSN 0022-2836. PMID 12614612.
↑ ^4,0 ^4,1 ^4,2 Yabuta, Norikazu; Kajimura Naoko; Mayanagi Kouta; Sato Michio; Gotow Takahito; Uchiyama Yasuo; Ishimi Yukio; Nojima Hiroshi (May 2003). „Mammalian Mcm2/4/6/7 complex forms a toroidal structure”. Genes Cells (England) 8 (5): 413–21. DOI:10.1046/j.1365-2443.2003.00645.x. ISSN 1356-9597. PMID 12694531.
↑ ^5,0 ^5,1 ^5,2 Ishimi, Y; Ichinose S; Omori A; Sato K; Kimura H (September 1996). „Binding of human minichromosome maintenance proteins with histone H3”. J. Biol. Chem. (UNITED STATES) 271 (39): 24115–22. DOI:10.1074/jbc.271.39.24115. ISSN 0021-9258. PMID 8798650.
↑ ^6,0 ^6,1 You, Zhiying; Ishimi Yukio; Masai Hisao; Hanaoka Fumio (November 2002). „Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complex”. J. Biol. Chem. (United States) 277 (45): 42471–9. DOI:10.1074/jbc.M205769200. ISSN 0021-9258. PMID 12207017.
↑ Fujita, M; Kiyono T; Hayashi Y; Ishibashi M (April 1997). „In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP”. J. Biol. Chem. (UNITED STATES) 272 (16): 10928–35. DOI:10.1074/jbc.272.16.10928. ISSN 0021-9258. PMID 9099751.
↑ You, Z; Komamura Y; Ishimi Y (December 1999). „Biochemical Analysis of the Intrinsic Mcm4-Mcm6-Mcm7 DNA Helicase Activity”. Mol. Cell. Biol. (UNITED STATES) 19 (12): 8003–15. ISSN 0270-7306. PMC 84885. PMID 10567526.

Literatura

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Schulte D, Richter A, Burkhart R i dr.. (1996). „Properties of the human nuclear protein p85Mcm. Expression, nuclear localization and interaction with other Mcm proteins”. Eur. J. Biochem. 235 (1–2): 144–51. DOI:10.1111/j.1432-1033.1996.00144.x. PMID 8631321.
Ishimi Y, Ichinose S, Omori A i dr.. (1996). „Binding of human minichromosome maintenance proteins with histone H3”. J. Biol. Chem. 271 (39): 24115–22. DOI:10.1074/jbc.271.39.24115. PMID 8798650.
Ladenburger EM, Fackelmayer FO, Hameister H, Knippers R (1997). „MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12→q13”. Cytogenet. Cell Genet. 77 (3–4): 268–70. DOI:10.1159/000134594. PMID 9284934.
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Kneissl M, Pütter V, Szalay AA, Grummt F (2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. 327 (1): 111–28. DOI:10.1016/S0022-2836(03)00079-2. PMID 12614612.
Yabuta N, Kajimura N, Mayanagi K i dr.. (2004). „Mammalian Mcm2/4/6/7 complex forms a toroidal structure”. Genes Cells 8 (5): 413–21. DOI:10.1046/j.1365-2443.2003.00645.x. PMID 12694531.
Ishimi Y, Komamura-Kohno Y, Kwon HJ i dr.. (2003). „Identification of MCM4 as a target of the DNA replication block checkpoint system”. J. Biol. Chem. 278 (27): 24644–50. DOI:10.1074/jbc.M213252200. PMID 12714602.
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