RFC3

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Replikacioni faktor C (aktivator 1) 3, 38kDa
Identifikatori
Simboli	RFC3; RFC38
Vanjski ID	OMIM: 600405 MGI: 1916513 HomoloGene: 2188 GeneCards: RFC3 Gene
Ontologija gena Molekulska funkcija • nukleotidno vezivanje • DNK vezivanje • aktivnost postavljača DNK stege Ćelijska komponenta • nukleoplazma • kompleks DNK replikacionog faktora C Biološki proces • kontrolno mesto ćelijskog ciklusa • S faza mitotičkog ćelijskog ciklusa • mitotički ćelijski ciklus
Pregled RNK izražavanja
podaci
Ortolozi
Vrsta	Čovek	Miš
Entrez	5983	69263
Ensembl	ENSG00000133119	ENSMUSG00000033970
UniProt	P40938	Q8R323
Ref. Sekv. (iRNK)	NM_002915	NM_027009
Ref. Sekv. (protein)	NP_002906	NP_081285
Lokacija (UCSC)	Chr 13: 34.39 - 34.54 Mb	Chr 5: 151.64 - 151.65 Mb
PubMed pretraga	[1]	[2]

Replikacioni faktor C, podjedinica 3 je protein koji je kod ljudi kodiran RFC3 genom.^[1][2]

Za elongaciju prajmiranog DNK šablona DNK polimerazom delta i DNK polimerazom epsilon neophodni su pomoćni proteini: proliferativni ćelijski nuklearni antigen (PCNA) i replikacioni faktor C (RFC). RFC, takođe poznat kao aktivator 1, je proteinski kompleks koji se sastoji od pet podjedinica sa 140, 40, 38, 37, i 36 kDa. Ovaj gen kodira 38 kDa podjednicu. Ta podjedinica je esencijalna za interakciju između 140 kDa podjedinice i srži kompleksa koja se sastoji od 36, 37, i 40 kDa podjedinica. Poznate su alternativno splajsovane transcriptne varijante koje kodiraju različite izoforme.^[2]

Interakcije

RFC3 formira interakcije sa BRD4,^[3] CHTF18,^[4][5] RFC1,^[6][7][8] PCNA^[5][9] i RFC4.^[6][7]

Reference

1. Okumura K, Nogami M, Taguchi H, Dean FB, Chen M, Pan ZQ, Hurwitz J, Shiratori A, Murakami Y, Ozawa K, et al. (Jul 1995). „Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23”. Genomics 25 (1): 274–8. DOI:10.1016/0888-7543(95)80135-9. PMID 7774928. 
2. „Entrez Gene: RFC3 replication factor C (activator 1) 3, 38kDa”. 
3. Maruyama, Tetsuo; Farina Andrea, Dey Anup, Cheong JaeHun, Bermudez Vladimir P, Tamura Tomohiko, Sciortino Selvaggia, Shuman Jon, Hurwitz Jerard, Ozato Keiko (Sep 2002). „A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase”. Mol. Cell. Biol. (United States) 22 (18): 6509–20. DOI:10.1128/MCB.22.18.6509-6520.2002. ISSN 0270-7306. PMC 135621. PMID 12192049. 
4. Bermudez, Vladimir P; Maniwa Yoshimasa, Tappin Inger, Ozato Keiko, Yokomori Kyoko, Hurwitz Jerard (Sep 2003). „The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA”. Proc. Natl. Acad. Sci. U.S.A. (United States) 100 (18): 10237–42. DOI:10.1073/pnas.1434308100. ISSN 0027-8424. PMC 193545. PMID 12930902. 
5. Merkle, Carolin J; Karnitz Larry M, Henry-Sánchez John T, Chen Junjie (Aug 2003). „Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment”. J. Biol. Chem. (United States) 278 (32): 30051–6. DOI:10.1074/jbc.M211591200. ISSN 0021-9258. PMID 12766176. 
6. Ellison, V; Stillman B (Mar 1998). „Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity”. J. Biol. Chem. (UNITED STATES) 273 (10): 5979–87. DOI:10.1074/jbc.273.10.5979. ISSN 0021-9258. PMID 9488738. 
7. Uhlmann, F; Cai J, Flores-Rozas H, Dean F B, Finkelstein J, O'Donnell M, Hurwitz J (Jun 1996). „In vitro reconstitution of human replication factor C from its five subunits”. Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 93 (13): 6521–6. DOI:10.1073/pnas.93.13.6521. ISSN 0027-8424. PMC 39056. PMID 8692848. 
8. Tomida, Junya; Masuda Yuji, Hiroaki Hidekazu, Ishikawa Tomoko, Song Ihnyoung, Tsurimoto Toshiki, Tateishi Satoshi, Shiomi Tadahiro, Kamei Yasuhiro, Kim Jinhyeong, Kamiya Kenji, Vaziri Cyrus, Ohmori Haruo, Todo Takeshi (Apr 2008). „DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex”. J. Biol. Chem. (United States) 283 (14): 9071–9. DOI:10.1074/jbc.M709835200. ISSN 0021-9258. PMC 2431014. PMID 18245774. 
9. Ohta, Satoshi; Shiomi Yasushi, Sugimoto Katsunori, Obuse Chikashi, Tsurimoto Toshiki (Oct 2002). „A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein”. J. Biol. Chem. (United States) 277 (43): 40362–7. DOI:10.1074/jbc.M206194200. ISSN 0021-9258. PMID 12171929. 

Literatura

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• O'Donnell M, Onrust R, Dean FB, et al. (1993). „Homology in accessory proteins of replicative polymerases--E. coli to humans.”. Nucleic Acids Res. 21 (1): 1–3. DOI:10.1093/nar/21.1.1. PMC 309057. PMID 8441605. 
• Uhlmann F, Cai J, Flores-Rozas H, et al. (1996). „In vitro reconstitution of human replication factor C from its five subunits.”. Proc. Natl. Acad. Sci. U.S.A. 93 (13): 6521–6. DOI:10.1073/pnas.93.13.6521. PMC 39056. PMID 8692848. 
• Uhlmann F, Cai J, Gibbs E, et al. (1997). „Deletion analysis of the large subunit p140 in human replication factor C reveals regions required for complex formation and replication activities.”. J. Biol. Chem. 272 (15): 10058–64. DOI:10.1074/jbc.272.15.10058. PMID 9092549. 
• Cujec TP, Cho H, Maldonado E, et al. (1997). „The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme.”. Mol. Cell. Biol. 17 (4): 1817–23. PMC 232028. PMID 9121429. 
• Cai J, Gibbs E, Uhlmann F, et al. (1997). „A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme.”. J. Biol. Chem. 272 (30): 18974–81. DOI:10.1074/jbc.272.30.18974. PMID 9228079. 
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). „Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.”. Gene 200 (1-2): 149–56. DOI:10.1016/S0378-1119(97)00411-3. PMID 9373149. 
• Ellison V, Stillman B (1998). „Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity.”. J. Biol. Chem. 273 (10): 5979–87. DOI:10.1074/jbc.273.10.5979. PMID 9488738. 
• Zhang G, Gibbs E, Kelman Z, et al. (1999). „Studies on the interactions between human replication factor C and human proliferating cell nuclear antigen.”. Proc. Natl. Acad. Sci. U.S.A. 96 (5): 1869–74. DOI:10.1073/pnas.96.5.1869. PMC 26703. PMID 10051561. 
• Rauen M, Burtelow MA, Dufault VM, Karnitz LM (2000). „The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9.”. J. Biol. Chem. 275 (38): 29767–71. DOI:10.1074/jbc.M005782200. PMID 10884395. 
• Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A (2001). „Purification and characterization of human DNA damage checkpoint Rad complexes.”. Proc. Natl. Acad. Sci. U.S.A. 98 (20): 11236–41. DOI:10.1073/pnas.201373498. PMC 58713. PMID 11572977. 
• Griffith JD, Lindsey-Boltz LA, Sancar A (2002). „Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy.”. J. Biol. Chem. 277 (18): 15233–6. DOI:10.1074/jbc.C200129200. PMID 11907025. 
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• Maruyama T, Farina A, Dey A, et al. (2002). „A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase.”. Mol. Cell. Biol. 22 (18): 6509–20. DOI:10.1128/MCB.22.18.6509-6520.2002. PMC 135621. PMID 12192049. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.”. Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. DOI:10.1073/pnas.242603899. PMC 139241. PMID 12477932. 
• Merkle CJ, Karnitz LM, Henry-Sánchez JT, Chen J (2003). „Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment.”. J. Biol. Chem. 278 (32): 30051–6. DOI:10.1074/jbc.M211591200. PMID 12766176. 
• Bermudez VP, Maniwa Y, Tappin I, et al. (2003). „The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA.”. Proc. Natl. Acad. Sci. U.S.A. 100 (18): 10237–42. DOI:10.1073/pnas.1434308100. PMC 193545. PMID 12930902. 
• Zou L, Liu D, Elledge SJ (2004). „Replication protein A-mediated recruitment and activation of Rad17 complexes.”. Proc. Natl. Acad. Sci. U.S.A. 100 (24): 13827–32. DOI:10.1073/pnas.2336100100. PMC 283506. PMID 14605214. 
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). „Complete sequencing and characterization of 21,243 full-length human cDNAs.”. Nat. Genet. 36 (1): 40–5. DOI:10.1038/ng1285. PMID 14702039.