TRNK guanozin-2'-O-metiltransferaza

TRNK guanozin-2'-O-metiltransferaza
Identifikatori
EC broj	2.1.1.34
CAS broj	37257-01-5
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

TRNK guanozin-2'-O-metiltransferaza (EC 2.1.1.34, tRNK (Gm18) 2'-O-metiltransferaza, tRNK (Gm18) metiltransferaza, TrmH, SpoU) je enzim sa sistematskim imenom S-adenozil-L-metionin:tRNK (guanozin18-2'-O)-metiltransferaza.^{[1][2][3][4][5]} Ovaj enzim katalizuje sledeću hemijsku reakciju

S-adenozil-L-metionin + guanozin¹⁸ u tRNK ${\displaystyle \rightleftharpoons }$ S-adenozil-L-homocistein + 2'-O-metilguanozin¹⁸ u tRNK

Ovaj enzim katalizuje metilaciju guanozina¹⁸ unutar tRNK.

Reference

1. Gefter, M.L. (1969). „The in vitro synthesis of 2′-O-methylguanosine and 2-methylthio ⁶N (γ,gamma-dimethylallyl) adenosine in transfer RNA of Escherichia coli”. Biochem. Biophys. Res. Commun. 36: 435-441. PMID 4898378. 
2. Kumagai, I., Watanabe, K. and Oshima, T. (1980). „Thermally induced biosynthesis of 2′-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27”. Proc. Natl. Acad. Sci. USA 77: 1922-1926. PMID 6990416. 
3. Hori, H., Yamazaki, N., Matsumoto, T., Watanabe, Y., Ueda, T., Nishikawa, K., Kumagai, I. and Watanabe, K. (1998). „Substrate recognition of tRNA (guanosine-2′-)-methyltransferase from Thermus thermophilus HB27”. J. Biol. Chem. 273: 25721-25727. PMID 9748240. 
4. Pleshe, E., Truesdell, J. and Batey, R.T. (2005). „Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus”. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61: 722-728. PMID 16511140. 
5. Ochi, A., Makabe, K., Kuwajima, K. and Hori, H. (2010). „Flexible recognition of the tRNA G18 methylation target site by TrmH methyltransferase through first binding and induced fit processes”. J. Biol. Chem. 285: 9018-9029. PMID 20053984. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH TRNA+(guanosine18-2'-O)-methyltransferase