TRNK (guanin26-N2)-dimetiltransferaza

TRNK (guanin^26-N^2)-dimetiltransferaza
Identifikatori
EC broj	2.1.1.216
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

TRNK (guanin²⁶-N²)-dimetiltransferaza (EC 2.1.1.216, Trm1p, TRM1, tRNK (m22G26)dimetiltransferaza) je enzim sa sistematskim imenom S-adenozil-L-metionin:tRNK (guanin²⁶-N²)-dimetiltransferaza.^[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju

2 S-adenozil-L-metionin + guanin²⁶ u tRNK ${\displaystyle \rightleftharpoons }$ 2 S-adenozil-L-homocistein + N²-dimetilguanin²⁶ u tRNK

Enzim se disocira od svog tRNK supstrata između dve konsekutivne reakcije metilacije. Za razliku od EC 2.1.1.215, tRNK (guanin²⁶-N²/guanin²⁷-N²)-dimetiltransferaze, ovaj enzim ne katalizuje metilaciju guanina²⁷ u tRNK.

Reference

1. Constantinesco, F., Motorin, Y. and Grosjean, H. (1999). „Characterisation and enzymatic properties of tRNA(guanine²⁶, N²,N²-dimethyltransferase (Trm1p) from Pyrococcus furiosus”. J. Mol. Biol. 291: 375-392. PMID 10438627. 
2. Constantinesco, F., Benachenhou, N., Motorin, Y. and Grosjean, H. (1998). „The tRNA(guanine-26,N²-N²) methyltransferase (Trm1) from the hyperthermophilic archaeon Pyrococcus furiosus: cloning, sequencing of the gene and its expression in Escherichia coli”. Nucleic Acids Res. 26: 3753-3761. PMID 9685492. 
3. Liu, J., Liu, J. and Straby, K.B. (1998). „Point and deletion mutations eliminate one or both methyl group transfers catalysed by the yeast TRM1 encoded tRNA (m²₂G₂₆)dimethyltransferase”. Nucleic Acids Res. 26: 5102-5108. PMID 9801306. 
4. Liu, J., Zhou, G.Q. and Straby, K.B. (1999). „Caenorhabditis elegans ZC376.5 encodes a tRNA (m²₂G₂₆)dimethyltransferance in which ²⁴⁶arginine is important for the enzyme activity”. Gene 226: 73-81. PMID 10048958. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH TRNA+(guanine26-N2)-dimethyltransferase