TRNK (guanin-N7-)-metiltransferaza

TRNK (guanin-N7-)-metiltransferaza
Identifikatori
EC broj	2.1.1.33
CAS broj	37257-00-4
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

TRNK (guanin-N7-)-metiltransferaza (EC 2.1.1.33, Trm8/Trm82, TrmB, tRNK (m7G46) metiltransferaza, transfer ribonukleat guaninska 7-metiltransferaza, 7-metilguanin transfer ribonukleatna metilaza, tRNK guanin 7-metiltransferaza, N7-metilguaninska metilaza, S-adenozil-L-metionin:tRNK (guanin-7-N-)-metiltransferaza) je enzim sa sistematskim imenom S-adenozil-L-metionin:tRNK (guanin-N7)-metiltransferaza.^{[1][2][3][4][5]} Ovaj enzim katalizuje sledeću hemijsku reakciju

S-adenozil-L-metionin + guanin⁴⁶ u tRNK ${\displaystyle \rightleftharpoons }$ S-adenozil-L-homocistein + N⁷-metilguanin⁴⁶ u tRNK

Ovaj enzim specifično metiliše guanin⁴⁶ u poziciji N⁷ unutar tRNK.

Reference

1. Aschhoff, H.J., Elten, H., Arnold, H.H., Mahal, G., Kersten, W. and Kersten, H. (1976). „7-Methylguanine specific tRNA-methyltransferase from Escherichia coli”. Nucleic Acids Res. 3: 3109-3122. PMID 794833. 
2. Zegers, I., Gigot, D., van Vliet, F., Tricot, C., Aymerich, S., Bujnicki, J.M., Kosinski, J. and Droogmans, L. (2006). „Crystal structure of Bacillus subtilis TrmB, the tRNA (m⁷G⁴⁶) methyltransferase”. Nucleic Acids Res. 34: 1925-1934. PMID 16600901. 
3. Purta, E., van Vliet, F., Tricot, C., De Bie, L.G., Feder, M., Skowronek, K., Droogmans, L. and Bujnicki, J.M. (2005). „Sequence-structure-function relationships of a tRNA (m⁷G⁴⁶) methyltransferase studied by homology modeling and site-directed mutagenesis”. Proteins 59: 482-488. PMID 15789416. 
4. Liu, Q., Gao, Y., Yang, W., Zhou, H., Gao, Y., Zhang, X., Teng, M. and Niu, L. (2008). „Crystallization and preliminary crystallographic analysis of tRNA (m⁷G46) methyltransferase from Escherichia coli”. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64: 743-745. PMID 18678947. 
5. Alexandrov, A., Martzen, M.R. and Phizicky, E.M. (2002). „Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA”. RNA 8: 1253-1266. PMID 12403464. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH TRNA+(guanine46-N7)-methyltransferase