Ent-kaurenoinska kiselina oksidaza

Ent-kaurenoinska kiselina oksidaza
Identifikatori
EC broj	1.14.13.79
CAS broj	337507-95-6
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Ent-kaurenoinska kiselina oksidaza (EC 1.14.13.79, ent-kaurenoinsko kiselinska oksidaza) je enzim sa sistematskim imenom ent-kaur-16-en-19-oat,NADPH:kiseonik oksidoreduktaza (hidroksilacija).^[1] Ovaj enzim katalizuje sledeću hemijsku reakciju

ent-kaur-16-en-19-oat + 3 NADPH + 3 H⁺ + 3 O₂ ${\displaystyle \rightleftharpoons }$ giberelin A12 + 3 NADP⁺ + 4H₂O (sveukupna reakcija)

(1a) ent-kaur-16-en-19-oat + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ ent-7alfa-hidroksikaur-16-en-19-oat + NADP⁺ +H₂O

(1b) ent-7alfa-hidroksikaur-16-en-19-oat + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ giberelin A12 aldehid + NADP⁺ + 2H₂O

(1c) giberelin A12 aldehid + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ giberelin A12 + NADP⁺ +H₂O

Za rad ovog enzima je neophodan citohrom P450.

Reference

1. Helliwell, C.A., Chandler, P.M., Poole, A., Dennis, E.S. and Peacock, W.J. (2001). „The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway”. Proc. Natl. Acad. Sci. USA 98: 2065-2070. PMID 11172076. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH Ent-kaurenoic+acid+oxidase