Dihloroarcirijaflavin A sintaza

Dihloroarcirijaflavin A sintaza
Identifikatori
EC broj	1.13.12.17
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Dihloroarcirijaflavin A sintaza (EC 1.13.12.17, dihloroarcirijaflavinska A sintaza) je enzim sa sistematskim imenom dihlorohromopirolat,NADH:kiseonik 2,5-oksidoreduktaza (formira dihloroarcirijaflavin A).^[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju

dihlorohromopirolat + 4 O₂ + 4 NADH + 4 H⁺ ${\displaystyle \rightleftharpoons }$ dihloroarcirijaflavin A + 2 CO₂ + 6H₂O + 4 NAD⁺

Konverzija dihlorohromopirolata u dihloroarcirijaflavin A je kompleksan process u kome učestvuju dve enzimske komponente. RebP je NAD-zavisna citohrom P450 oksigenaza koja izvodi formiranje aril-aril veze čime se formira šestočlani prsten indolokarbazola. Zajedno sa RebC, flavin-zavisnom hidroksilaza, on takođe katalizuje oksidativnu dekarboksilaciju obe karboksilne grupe.

Reference

1. Makino, M., Sugimoto, H., Shiro, Y., Asamizu, S., Onaka, H. and Nagano, S. (2007). „Crystal structures and catalytic mechanism of cytochrome P₄₅₀ StaP that produces the indolocarbazole skeleton”. Proc. Natl. Acad. Sci. USA 104: 11591-11596. PMID 17606921. 
2. Howard-Jones, A.R. and Walsh, C.T. (2006). „Staurosporine and rebeccamycin aglycones are assembled by the oxidative action of StaP, StaC, and RebC on chromopyrrolic acid”. J. Am. Chem. Soc. 128: 12289-12298. PMID 16967980. 
3. Sanchez, C., Zhu, L., Brana, A.F., Salas, A.P., Rohr, J., Mendez, C. and Salas, J.A. (2005). „Combinatorial biosynthesis of antitumor indolocarbazole compounds”. Proc. Natl. Acad. Sci. USA 102: #461-466. PMID 15625109. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH Dichloroarcyriaflavin+A+synthase