CMP-N-acetilneuraminat monooksigenaza

CMP-N-acetilneuraminat monooksigenaza
Identifikatori
EC broj	1.14.18.2
CAS broj	116036-67-0
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

CMP-N-acetilneuraminat monooksigenaza (EC 1.14.18.2, CMP-N-acetilneuraminska kiselina hidroksilaza, CMP-Neu5Ac hidroksilaza, citidin monofosfoacetilneuraminatna monooksigenaza, N-acetilneuraminska monooksigenaza, citidin-5'-monofosfat-N-acetilneuraminska kiselina hidroksilaza) je enzim sa sistematskim imenom CMP-N-acetilneuraminat,ferocitohrom-b5:kiseonik oksidoreduktaza (N-acetil-hidroksilacija).^{[1][2][3][4][5]} Ovaj enzim katalizuje sledeću hemijsku reakciju

CMP-N-acetilneuraminat + 2 ferocitohrom b5 + O₂ + 2 H⁺ ${\displaystyle \rightleftharpoons }$ CMP-N-glikoloilneuraminat + 2 fericitohrom b5 + H₂O

Ovaj enzim sadrži Riskijev tip [2Fe-2S] klastera i jedan dodatno mesto vezivanja gvožđa.

Reference

1. Shaw, L. and Schauer, R. (1988). „The biosynthesis of N-glycoloylneuraminic acid occurs by hydroxylation of the CMP-glycoside of N-acetylneuraminic acid”. Biol. Chem. Hoppe-Seyler 369: 477-486. PMID 3202954. 
2. Kozutsumi, Y., Kawano, T., Yamakawa, T. and Suzuki, A. (1990). „Participation of cytochrome b₅ in CMP-N-acetylneuraminic acid hydroxylation in mouse liver cytosol”. J. Biochem. (Tokyo) 109: 704-706. PMID 1964451. 
3. Schneckenburger, P., Shaw, L. and Schauer, R. (1994). „Purification, characterization and reconstitution of CMP-N-acetylneuraminate hydroxylase from mouse liver”. Glycoconj. J. 11: 194-203. PMID 7841794. 
4. Kawano, T., Koyama, S., Takematsu, H., Kozutsumi, Y., Kawasaki, H., Kawashima, S., Kawasaki, T. and Suzuki, A. (1995). „Molecular cloning of cytidine monophospho-N-acetylneuraminic acid hydroxylase. Regulation of species- and tissue-specific expression of N-glycolylneuraminic acid”. J. Biol. Chem. 270: 16458-16463. PMID 7608218. 
5. Schlenzka, W., Shaw, L., Kelm, S., Schmidt, C.L., Bill, E., Trautwein, A.X., Lottspeich, F. and Schauer, R. (1996). „CMP-N-acetylneuraminic acid hydroxylase: the first cytosolic Rieske iron-sulphur protein to be described in Eukarya”. FEBS Lett. 385: 197-200. PMID 8647250. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH CMP-N-acetylneuraminate+monooxygenase