4-hidroksitreonin-4-fosfat dehidrogenaza

4-hidroksitreonin-4-fosfat dehidrogenaza
Identifikatori
EC broj	1.1.1.262
CAS broj	230310-36-8
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

4-hidroksitreonin-4-fosfat dehidrogenaza (EC 1.1.1.262, NAD⁺-zavisni treonin 4-fosfat dehidrogenaza, L-treonin 4-fosfat dehidrogenaza, 4-(fosfohidroksi)-L-treonin dehidrogenaza, PdxA, 4-(fosfonooksi)-L-treonin:NAD⁺ oksidoreduktaza) je enzim sa sistematskim imenom 4-fosfonooksi-L-treonin:NAD⁺ oksidoreduktaza.^[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju

4-fosfonooksi-L-treonin + NAD⁺ ${\displaystyle \rightleftharpoons }$ 3-amino-2-oksopropil fosfat + CO₂ + NADH + H⁺ (sveukupna reakcija)

(1a) 4-fosfonooksi-L-treonin + NAD⁺ ${\displaystyle \rightleftharpoons }$ (2S)-2-amino-3-okso-4-fosfonooksibutanoat + NADH + H⁺

(1b) (2S)-2-amino-3-okso-4-fosfonooksibutanoat ${\displaystyle \rightleftharpoons }$ 3-amino-2-oksopropil fosfat + CO₂ (spontana reakcija)

Product reakcije podleže dekarboksilaciji, čime se formira 3-amino-2-oksopropil fosfat. Ovaj enzim je deo biosinetičkog puta koenzima piridoksal 5'-fosfat prusutnog kod anaerobnih bakterija.

Reference

1. Cane, D.E., Hsiung, Y., Cornish, J.A., Robinson, J.K and Spenser, I.D. (1998). „Biosynthesis of vitamine B₆: The oxidation of L-threonine 4-phosphate by PdxA”. J. Am. Chem. Soc. 120: 1936-1937. 
2. Laber, B., Maurer, W., Scharf, S., Stepusin, K. and Schmidt, F.S. (1999). „Vitamin B₆ biosynthesis: formation of pyridoxine 5′-phosphate from 4-(phosphohydroxy)-L-threonine and 1-deoxy-D-xylulose-5-phosphate by PdxA and PdxJ protein”. FEBS Lett. 449: 45-48. PMID 10225425. 
3. Sivaraman, J., Li, Y., Banks, J., Cane, D.E., Matte, A. and Cygler, M. (2003). „Crystal structure of Escherichia coli PdxA, an enzyme involved in the pyridoxal phosphate biosynthesis pathway”. J. Biol. Chem. 278: 43682-43690. PMID 12896974. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH 4-hydroxythreonine-4-phosphate+dehydrogenase