23S rRNA (adenine2503-C2)-methyltransferase

23S rRNA (adenine^2503-C^2)-methyltransferase
Identifiers
EC no.	2.1.1.192
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

23S rRNA (adenine²⁵⁰³-C²)-methyltransferase (EC 2.1.1.192, RlmN, YfgB, Cfr) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenine²⁵⁰³-C²)-methyltransferase.^{[1][2][3][4][5]} This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + adenine²⁵⁰³ in 23S rRNA ${\displaystyle \rightleftharpoons }$ ^{S-adenosyl-L-homocysteine} + L-methionine + 5'-deoxyadenosine + 2-methyladenine²⁵⁰³ in 23S rRNA

23S rRNA (adenine²⁵⁰³-C²)-methyltransferase contains an [4Fe-4S] cluster.

References

1. Toh SM, Xiong L, Bae T, Mankin AS (January 2008). "The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA". RNA. 14 (1): 98–106. doi:10.1261/rna.814408. PMC 2151032. PMID 18025251.
2. Yan F, LaMarre JM, Röhrich R, Wiesner J, Jomaa H, Mankin AS, Fujimori DG (March 2010). "RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA". Journal of the American Chemical Society. 132 (11): 3953–64. doi:10.1021/ja910850y. PMC 2859901. PMID 20184321.
3. Yan F, Fujimori DG (March 2011). "RNA methylation by radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift". Proceedings of the National Academy of Sciences of the United States of America. 108 (10): 3930–4. Bibcode:2011PNAS..108.3930Y. doi:10.1073/pnas.1017781108. PMC 3054002. PMID 21368151.
4. Grove TL, Benner JS, Radle MI, Ahlum JH, Landgraf BJ, Krebs C, Booker SJ (April 2011). "A radically different mechanism for S-adenosylmethionine-dependent methyltransferases". Science. 332 (6029): 604–7. Bibcode:2011Sci...332..604G. doi:10.1126/science.1200877. PMID 21415317. S2CID 2214309.
5. Boal AK, Grove TL, McLaughlin MI, Yennawar NH, Booker SJ, Rosenzweig AC (May 2011). "Structural basis for methyl transfer by a radical SAM enzyme". Science. 332 (6033): 1089–92. Bibcode:2011Sci...332.1089B. doi:10.1126/science.1205358. PMC 3506250. PMID 21527678.

External links

• 23S+rRNA+(adenine2503-C2)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)