2-oxoisovalerate dehydrogenase (acylating)

2-oxoisovalerate dehydrogenase (acylating)
Identifiers
EC no.	1.2.1.25
CAS no.	37211-61-3
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a 2-oxoisovalerate dehydrogenase (acylating) (EC 1.2.1.25) is an enzyme that catalyzes the chemical reaction

3-methyl-2-oxobutanoate + CoA + NAD⁺ ${\displaystyle \rightleftharpoons }$ 2-methylpropanoyl-CoA + CO₂ + NADH

The 3 substrates of this enzyme are 3-methyl-2-oxobutanoate, CoA, and NAD⁺, whereas its 3 products are 2-methylpropanoyl-CoA, CO₂, and NADH.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 3-methyl-2-oxobutanoate:NAD+ 2-oxidoreductase (CoA-methyl-propanoylating). Other names in common use include 2-oxoisovalerate dehydrogenase, and alpha-ketoisovalerate dehydrogenase. This enzyme participates in valine, leucine and isoleucine degradation.

References

• Namba Y, Yoshizawa K, Ejima A, Hayashi T, Kaneda T (1969). "Coenzyme A- and nicotinamide adenine dinucleotide-dependent branched chain alpha-keto acid dehydrogenase. I. Purification and properties of the enzyme from Bacillus subtilis". J. Biol. Chem. 244 (16): 4437–47. PMID 4308861.