Taurohenodezoksiholat 6α-hidroksilaza

Taurohenodezoksiholat 6α-hidroksilaza
Identifikatori
EC broj	1.14.13.97
CAS broj	105669-85-0
Baze podataka
IntEnz	IntEnz pregled
BRENDA	BRENDA pristup
ExPASy	NiceZyme pregled
KEGG	KEGG pristup
MetaCyc	metabolički put
PRIAM	profil
Strukture PBP	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC članci PubMed članci NCBI proteini

Taurohenodezoksiholat 6α-hidroksilaza (EC 1.14.13.97, CYP3A4, CYP4A21, taurohenodezoksiholat 6alfa-monooksigenaza) je enzim sa sistematskim imenom taurohenodezoksiholat,NADPH:kiseonik oksidoreduktaza (6alfa-hidroksilacija).^{[1][2][3][4][5][6]} Ovaj enzim katalizuje sledeću hemijsku reakciju

(1) taurohenodezoksiholat + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ taurohioholat + NADP⁺ + H₂O

(2) lithoholat + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ hiodezoksiholat + NADP⁺ + H₂O

Ovaj enzim je hem-tiolatni protein (P-450). Za maksimalnu aktivnost je neophodan citohrom b5.

Reference

1. Araya, Z. & Wikvall, K. (1999). „6α-Hydroxylation of taurochenodeoxycholic acid and lithocholic acid by CYP3A4 in human liver microsomes”. Biochim. Biophys. Acta. 1438: 47—54. PMID 10216279. 
2. Araya, Z., Hellman, U. and Hansson, R. (1995). „Characterisation of taurochenodeoxycholic acid 6α-hydroxylase from pig liver microsomes”. Eur. J. Biochem. 231: 855—861. PMID 7649186. CS1 одржавање: Вишеструка имена: списак аутора (веза)
3. Kramer, W., Sauber, K., Baringhaus, K.H., Kurz, M., Stengelin, S., Lange, G., Corsiero, D., Girbig, F., Konig, W. and Weyland, C. (2001). „Identification of the bile acid-binding site of the ileal lipid-binding protein by photoaffinity labeling, matrix-assisted laser desorption ionization-mass spectrometry, and NMR structure”. J. Biol. Chem. 276: 7291—7301. PMID 11069906. CS1 одржавање: Вишеструка имена: списак аутора (веза)
4. Lundell, K., Hansson, R. and Wikvall, K. (2001). „Cloning and expression of a pig liver taurochenodeoxycholic acid 6α-hydroxylase (CYP4A21): a novel member of the CYP4A subfamily”. J. Biol. Chem. 276: 9606—9612. PMID 11113117. CS1 одржавање: Вишеструка имена: списак аутора (веза)
5. Lundell, K. & Wikvall, K. (2003). „Gene structure of pig sterol 12α-hydroxylase (CYP8B1) and expression in fetal liver: comparison with expression of taurochenodeoxycholic acid 6α-hydroxylase (CYP4A21)”. Biochim. Biophys. Acta. 1634: 86—96. PMID 14643796. 
6. Russell, D.W. (2003). „The enzymes, regulation, and genetics of bile acid synthesis”. Annu. Rev. Biochem. 72: 137—174. PMID 12543708. 

Literatura

• Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036. 
• Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097. 
• William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605. 

Spoljašnje veze

• Taurochenodeoxycholate+6alpha-hydroxylase на US National Library of Medicine Medical Subject Headings (MeSH)