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Pretraga
PMC	članci
PubMed	članci
NCBI	proteini

Kaspaza-3
Kaspaza-3
Identifikatori
EC broj	3.4.22.56
CAS broj	169592-56-7
Baze podataka
IntEnz	IntEnz pregled
BRENDA	BRENDA pristup
ExPASy	NiceZyme pregled
KEGG	KEGG pristup
MetaCyc	metabolički put
PRIAM	profil
Strukture PBP	RCSB PDB PDBe PDBj PDBsum
PMC
Pretraga
PMC	članci
PubMed	članci
NCBI	proteini

Kaspaza-3 (EC 3.4.22.56, CPP32, apopain, jama protein) je enzim.^[1]^[2]^[3]^[4]^[5]^[6] Ovaj enzim katalizuje sledeću hemijsku reakciju

Neophodno je prisustvo Asp ostatka u pozicijama P1 i P4. Preferentno dolazi do razlaganja sekvence Asp-Xaa-Xaa-Asp- sa hidrofobnim aminokiselinskim ostatkom u P2 poziciji i hidrofilnim aminokiselinskim ostatkom u P3, mada Val ili Ala takođe mogu da budu u toj poziciji

Kaspaza-3 je efektor/izvršilac kaspaze, kao što su i kaspaza-6 (EC 3.4.22.59) i kaspaza-7 (EC 3.4.22.60).

Reference

^ Krebs, J.F., Srinivasan, A., Wong, A.M., Tomaselli, K.J., Fritz, L.C. and Wu, J.C. (2000). „Heavy membrane-associated caspase 3: identification, isolation, and characterization”. Biochemistry. 39: 16056—16063. PMID 11123933.
^ Li, H., Bergeron, L., Cryns, V., Pasternack, M.S., Zhu, H., Shi, L., Greenberg, A. and Yuan, J. (1997). „Activation of caspase-2 in apoptosis”. J. Biol. Chem. 272: 21010—21017. PMID 9261102.
^ Nicholson, D. & Thornberry, N.A. (2004). „Caspase-3 and caspase-7”. Ур.: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. Handbook of Proteolytic Enzymes (2nd изд.). London: Elsevier. стр. 1298—1302.
^ Fang, B., Boross, P.I., Tozser, J. and Weber, I.T. (2006). „Structural and kinetic analysis of caspase-3 reveals role for S5 binding site in substrate recognition”. J. Mol. Biol. 360: 654—666. PMID 16781734.
^ Chang, H.Y. & Yang, X. (2000). „Proteases for cell suicide: functions and regulation of caspases”. Microbiol. Mol. Biol. Rev. 64: 821—846. PMID 11104820.
^ Martin, S.J., Amarante-Mendes, G.P., Shi, L., Chuang, T.H., Casiano, C.A., O'Brien, G.A., Fitzgerald, P., Tan, E.M., Bokoch, G.M., Greenberg, A.H. and Green, D.R. (1996). „The cytotoxic cell protease granzyme B initiates apoptosis in a cell-free system by proteolytic processing and activation of the ICE/CED-3 family protease, CPP32, via a novel two-step mechanism”. EMBO J. 15: 2407—2416. PMID 8665848.

Literatura

Nicholson, D. & Thornberry, N.A. (2004). „Caspase-3 and caspase-7”. Ур.: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. Handbook of Proteolytic Enzymes (2nd изд.). London: Elsevier. стр. 1298—1302.

Spoljašnje veze

Caspase-3 на US National Library of Medicine Medical Subject Headings (MeSH)

[1] Krebs, J.F., Srinivasan, A., Wong, A.M., Tomaselli, K.J., Fritz, L.C. and Wu, J.C. (2000). „Heavy membrane-associated caspase 3: identification, isolation, and characterization”. Biochemistry. 39: 16056—16063. PMID 11123933.

[2] Li, H., Bergeron, L., Cryns, V., Pasternack, M.S., Zhu, H., Shi, L., Greenberg, A. and Yuan, J. (1997). „Activation of caspase-2 in apoptosis”. J. Biol. Chem. 272: 21010—21017. PMID 9261102.

[3] Nicholson, D. & Thornberry, N.A. (2004). „Caspase-3 and caspase-7”. Ур.: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. Handbook of Proteolytic Enzymes (2nd изд.). London: Elsevier. стр. 1298—1302.

[4] Fang, B., Boross, P.I., Tozser, J. and Weber, I.T. (2006). „Structural and kinetic analysis of caspase-3 reveals role for S5 binding site in substrate recognition”. J. Mol. Biol. 360: 654—666. PMID 16781734.

[5] Chang, H.Y. & Yang, X. (2000). „Proteases for cell suicide: functions and regulation of caspases”. Microbiol. Mol. Biol. Rev. 64: 821—846. PMID 11104820.

[6] Martin, S.J., Amarante-Mendes, G.P., Shi, L., Chuang, T.H., Casiano, C.A., O'Brien, G.A., Fitzgerald, P., Tan, E.M., Bokoch, G.M., Greenberg, A.H. and Green, D.R. (1996). „The cytotoxic cell protease granzyme B initiates apoptosis in a cell-free system by proteolytic processing and activation of the ICE/CED-3 family protease, CPP32, via a novel two-step mechanism”. EMBO J. 15: 2407—2416. PMID 8665848.

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