Dolihil-P-Glc:Glc1Man9GlcNAc2-PP-dolihol alfa-1,3-glukoziltransferaza

Dolihil-P-Glc:Glc1Man9GlcNAc2-PP-dolihol alfa-1,3-glukoziltransferaza
Identifikatori
EC broj2.4.1.265
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

Dolihil-P-Glc:Glc1Man9GlcNAc2-PP-dolihol alfa-1,3-glukoziltransferaza (EC 2.4.1.265, ALG8, Dol-P-Glc:Glc1Man9GlcNAc2-PP-Dol alfa-1,3-glukoziltransferaza) je enzim sa sistematskim imenom dolihil beta-D-glukozil fosfat:D-Glc-alfa-(1->3)-D-Man-alfa-(1->2)-D-Man-alfa-(1->2)-D-Man-alfa-(1->3)-(D-Man-alfa-(1->2)-D-Man-alfa-(1->3)-(D-Man-alfa-(1->2)-D-Man-alfa-(1->6))-D-Man-alfa-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-difosfodolihol alfa-1,3-glukoziltransferaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju

dolihil beta-D-glukozil fosfat + D-Glc-alfa-(1->3)-D-Man-alfa-(1->2)-D-Man-alfa-(1->2)-D-Man-alfa-(1->3)-[D-Man-alfa-(1->2)-D-Man-alfa-(1->3)-[D-Man-alfa-(1->2)-D-Man-alfa-(1->6)]-D-Man-alfa-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-difosfodolihol D-Glc-alfa-(1->3)-D-Glc-alfa-(1->3)-D-Man-alfa-(1->2)-D-Man-alfa-(1->2)-D-Man-alfa-(1->3)-[D-Man-alfa-(1->2)-D-Man-alfa-(1->3)-[D-Man-alfa-(1->2)-D-Man-alfa-(1->6)]-D-Man-alfa-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-difosfodolihol + dolihil fosfat

Konsekutivna adicija tri glukozna ostatka posredstvom enzima EC 2.4.1.267 (dolihil-P-Glc:Man9GlcNAc2-PP-dolihol alfa-1,3-glukoziltransferaza), EC 2.4.1.265 i EC 2.4.1.256 (dolihil-P-Glc:Glc2Man9GlcNAc2-PP-dolihol alfa-1,2-glukoziltransferaza) predstavlja finalni korak formiranja lipid-vezanog oligosaharidnog sklopa.

Reference

  1. ^ Stagljar, I., te Heesen, S. and Aebi, M. (1994). „New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: cloning of the ALG8 locus”. Proc. Natl. Acad. Sci. USA. 91: 5977—5981. PMID 8016100. 
  2. ^ Runge, K.W. & Robbins, P.W. (1986). „A new yeast mutation in the glucosylation steps of the asparagine-linked glycosylation pathway. Formation of a novel asparagine-linked oligosaccharide containing two glucose residues”. J. Biol. Chem. 261: 15582—15590. PMID 3536907. 
  3. ^ Chantret, I., Dancourt, J., Dupre, T., Delenda, C., Bucher, S., Vuillaumier-Barrot, S., Ogier de Baulny, H., Peletan, C., Danos, O., Seta, N., Durand, G., Oriol, R., Codogno, P. and Moore, S.E. (2003). „A deficiency in dolichyl-P-glucose:Glc1Man9GlcNAc2-PP-dolichyl α3-glucosyltransferase defines a new subtype of congenital disorders of glycosylation”. J. Biol. Chem. 278: 9962—9971. PMID 12480927. 

Literatura

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