TRNK (guanin37-N1)-metiltransferaza

TRNK (guanin^37-N^1)-metiltransferaza
Identifikatori
EC broj	2.1.1.228
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

TRNK (guanin³⁷-N¹)-metiltransferaza (EC 2.1.1.228, TrmD, tRNK (m1G37) metiltransferaza, transfer RNK (m1G37) metiltransferaza, Trm5p, TRMT5, tRNK-(N1G37) metiltransferaza, MJ0883 (gen)) je enzim sa sistematskim imenom S-adenozil-L-metionin:tRNK (guanin³⁷-N¹)-metiltransferaza.^{[1][2][3][4][5][6]} Ovaj enzim katalizuje sledeću hemijsku reakciju

S-adenozil-L-metionin + guanin³⁷ u tRNK ${\displaystyle \rightleftharpoons }$ S-adenozil-L-homocistein + N¹-metilguanin³⁷ u tRNK

Ovaj enzim je važan za održavanje korektnog okvira čitanja tokom translacije. Za razliku od TrmD iz Escherichia coli, koji preferentno prepoznaje G36pG37 motif, ljudski enzim (kodiran TRMT5 genom) takođe metiluje inozin u poziciji 37.

Reference

1. Takeda, H., Toyooka, T., Ikeuchi, Y., Yokobori, S., Okadome, K., Takano, F., Oshima, T., Suzuki, T., Endo, Y. and Hori, H. (2006). „The substrate specificity of tRNA (m¹G³⁷) methyltransferase (TrmD) from Aquifex aeolicus”. Genes Cells 11: 1353-1365. PMID 17121543. 
2. Lee, C., Kramer, G., Graham, D.E. and Appling, D.R. (2007). „Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N¹-)-methyltransferase”. J. Biol. Chem. 282: 27744-27753. PMID 17652090. 
3. O'Dwyer, K., Watts, J.M., Biswas, S., Ambrad, J., Barber, M., Brule, H., Petit, C., Holmes, D.J., Zalacain, M. and Holmes, W.M. (2004). „Characterization of Streptococcus pneumoniae TrmD, a tRNA methyltransferase essential for growth”. J. Bacteriol. 186: 2346-2354. PMID 15060037. 
4. Brule, H., Elliott, M., Redlak, M., Zehner, Z.E. and Holmes, W.M. (2004). „Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein”. Biochemistry 43: 9243-9255. PMID 15248782. 
5. Goto-Ito, S., Ito, T., Ishii, R., Muto, Y., Bessho, Y. and Yokoyama, S. (2008). „Crystal structure of archaeal RNA(m¹G37)methyltransferase aTrm5”. Proteins 72: 1274-1289. PMID 18384044. 
6. Ahn, H.J., Kim, H.W., Yoon, H.J., Lee, B.I., Suh, S.W. and Yang, J.K. (2003). „Crystal structure of tRNA(m¹G³⁷)methyltransferase: insights into tRNA recognition”. EMBO J. 22: 2593-2603. PMID 12773376. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH TRNA+(guanine37-N1)-methyltransferase