TRNKHis guanililtransferaza

TRNKHis guanililtransferaza
Identifikatori
EC broj	2.7.7.79
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

TRNKHis guanililtransferaza (EC 2.7.7.79, histidin tRNK guanililtransferaza, Thg1p, Thg1) je enzim sa sistematskim imenom p-tRNKHis:GTP guanililtransferaza (ATP-hidroliza).^{[1][2][3][4][5][6]} Ovaj enzim katalizuje sledeću hemijsku reakciju

p-tRNKHis + ATP + GTP ${\displaystyle \rightleftharpoons }$ pppGp-tRNKHis + AMP + difosfat (sveukupna reakcija)

(1a) p-tRNKHis + ATP ${\displaystyle \rightleftharpoons }$ App-tRNKHis + difosfat

(1b) App-tRNKHis + GTP ${\displaystyle \rightleftharpoons }$ pppGp-tRNKHis + AMP

Kod eukariota jedan dodatni guanozinski ostatak je dodata posttranskripciono na 5'-kraj tRNKHis molekula.

Reference

1. Jahn, D. and Pande, S. (1991). „Histidine tRNA guanylyltransferase from Saccharomyces cerevisiae. II. Catalytic mechanism”. J. Biol. Chem. 266: 22832-22836. PMID 1660462. 
2. Pande, S., Jahn, D. and Soll, D. (1991). „Histidine tRNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and physical properties”. J. Biol. Chem. 266: 22826-22831. PMID 1660461. 
3. Gu, W., Jackman, J.E., Lohan, A.J., Gray, M.W. and Phizicky, E.M. (2003). „tRNA^His maturation: an essential yeast protein catalyzes addition of a guanine nucleotide to the 5′ end of tRNA^His”. Genes Dev. 17: 2889-2901. PMID 14633974. 
4. Placido, A., Sieber, F., Gobert, A., Gallerani, R., Giege, P. and Marechal-Drouard, L. (2010). „Plant mitochondria use two pathways for the biogenesis of tRNA^His”. Nucleic Acids Res. 38: 7711-7717. PMID 20660484. 
5. Jackman, J.E. and Phizicky, E.M. (2008). „Identification of critical residues for G-1 addition and substrate recognition by tRNA(His) guanylyltransferase”. Biochemistry 47: 4817-4825. PMID 18366186. 
6. Hyde, S.J., Eckenroth, B.E., Smith, B.A., Eberley, W.A., Heintz, N.H., Jackman, J.E. and Doublie, S. (2010). „tRNA(His) guanylyltransferase (THG1), a unique 3′-5′ nucleotidyl transferase, shares unexpected structural homology with canonical 5′-3′ DNA polymerases”. Proc. Natl. Acad. Sci. USA 107: 20305-20310. PMID 21059936. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH TRNAHis+guanylyltransferase