TRNK-pseudouridinska sintaza I

TRNK-pseudouridinska sintaza I
Identifikatori
EC broj	5.4.99.12
CAS broj	61506-89-6
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

TRNK-pseudouridinska sintaza I (EC 5.4.99.12, TruA, tRNK pseudouridinska sintaza I, PSUI, hisT (gen)) je enzim sa sistematskim imenom tRNK-uridin38-40 uracil mutaza.^{[1][2][3][4][5][6][7][8]} Ovaj enzim katalizuje sledeću hemijsku reakciju

tRNK uridin^38-40 ${\displaystyle \rightleftharpoons }$ tRNK pseudouridin^38-40

Uridilatni ostaci u pozicijama 38, 39 i 40 kod skoro svih tRNK molekula su izomerizovane do pseudouridina.

Reference

1. Hur, S. and Stroud, R.M. (2007). „How U38, 39, and 40 of many tRNAs become the targets for pseudouridylation by TruA”. Mol. Cell 26: 189-203. PMID 17466622. 
2. Huang, L., Pookanjanatavip, M., Gu, X. and Santi, D.V. (1998). „A conserved aspartate of tRNA pseudouridine synthase is essential for activity and a probable nucleophilic catalyst”. Biochemistry 37: 344-351. PMID 9425056. 
3. Kammen, H.O., Marvel, C.C., Hardy, L. and Penhoet, E.E. (1988). „Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I”. J. Biol. Chem. 263: 2255-2263. PMID 3276686. 
4. Turnbough, C.L., Jr., Neill, R.J., Landsberg, R. and Ames, B.N. (1979). „Pseudouridylation of tRNAs and its role in regulation in Salmonella typhimurium”. J. Biol. Chem. 254: 5111-5119. PMID 376505. 
5. Zhao, X. and Horne, D.A. (1997). „The role of cysteine residues in the rearrangement of uridine to pseudouridine catalyzed by pseudouridine synthase I”. J. Biol. Chem. 272: 1950-1955. PMID 8999885. 
6. Foster, P.G., Huang, L., Santi, D.V. and Stroud, R.M. (2000). „The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I”. Nat. Struct. Biol. 7: 23-27. PMID 10625422. 
7. Dong, X., Bessho, Y., Shibata, R., Nishimoto, M., Shirouzu, M., Kuramitsu, S. and Yokoyama, S. (2006). „Crystal structure of tRNA pseudouridine synthase TruA from Thermus thermophilus HB8”. RNA Biol. 3: 115-122. PMID 17114947. 
8. Arena, F., Ciliberto, G., Ciampi, S. and Cortese, R. (1978). „Purification of pseudouridylate synthetase I from Salmonella typhimurium”. Nucleic Acids Res. 5: 4523-4536. PMID 370771. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH TRNA+pseudouridine38-40+synthase