Pepsin-A

Pepsin-A
Pepsin, Human
Identifikatori
EC broj	3.4.23.1
CAS broj	9001-75-6
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Pepsin-A (EC 3.4.23.1, pepsin, laktatedni pepsin, pepsinski fortior, fundus-pepsin, eliksir laktatnog pepsina, P I, laktatedno pepsinski eliksir, P II, pepsin R, pepsin D) je enzim.^{[1][2][3][4][5][6][7]} Ovaj enzim katalizuje sledeću hemijsku reakciju

Preferentno razlaganje: hidrofobnih, preferentno aromatičnih, ostataka u P1 i P1' pozicijama. Razlaganje Phe¹-Val, Gln⁴-His, Glu¹³-Ala, Ala¹⁴-Leu, Leu¹⁵-Tyr, Tyr¹⁶-Leu, Gly²³-Phe, Phe²⁴-Phe i Phe²⁵-Tyr veza u B lancima insulina

Ovaj enzim je predominantna endopeptidaza u gastričnom soku kičmenjaka.

Reference

1. Lee, D. and Ryle, A.P. (1967). „Pepsinogen D. A fourth proteolytic zymogen from pig gastric mucosa”. Biochem. J. 104: 735-741. PMID 4167464. 
2. Lee, D. and Ryle, A.P. (1967). „Pepsin D. A minor component of commercial pepsin preparations”. Biochem. J. 104: 742-748. PMID 4860638. 
3. Foltmann, R. (1981). „Gastric proteinases -structure, function, evolution and mechanism of action”. Essays Biochem. 17: 52-84. PMID 6795036. 
4. James, M.N.G. and Sielecki, A.R. (1986). „Molecular structure of an aspartic proteinase zymogen, porcine pepsinogen, at 1.8 Å resolution”. Nature 319: 33-38. PMID 3941737. 
5. Fruton, J.S. (1987). „Aspartyl proteinases”. u: Neuberger, A. and Brocklehurst, K.. New Comprehensive Biochemistry: Hydrolytic Enzymes. 16. Amsterdam: Elsevier. str. 1-38. 
6. Tang, J. and Wong, R.N.S. (1987). „Evolution in the structure and function of aspartic proteases”. J. Cell. Biochem. 33: 53-63. PMID 3546346. 
7. Pohl, J. and Dunn, B.M. (1988). „Secondary enzyme-substrate interactions: kinetic evidence for ionic interactions between substrate side chains and the pepsin active site”. Biochemistry 27: 4827-4834. PMID 3139029. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

• Fruton, J.S. (1987). „Aspartyl proteinases”. u: Neuberger, A. and Brocklehurst, K.. New Comprehensive Biochemistry: Hydrolytic Enzymes. 16. Amsterdam: Elsevier. str. 1-38. 

Vanjske veze

• MeSH Pepsin+A