Nitrogenaza

Nitrogenaza
Identifikatori
EC broj	1.18.6.1
CAS broj	2598062
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Nitrogenaza (EC 1.18.6.1, Nitrogenase) je enzim sa sistematskim imenom redukovani feredoksin:dinitrogen oksidoreduktaza (ATP-hidroliza).^[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju

8 redukovani feredoksin + 8 H⁺ + N² + 16 ATP + 16H₂O ${\displaystyle \rightleftharpoons }$ 8 oksidovani feredoksin + H₂ + 2 NH₃ + 16 ADP + 16 fosfat

Za dejstvo ovog enzim je neohodan Mg²⁺. On se sastoji od dva proteina. Oni se mogu razdvojiti. Oba proteina su neophodna za nitrogenazno dejstvo.

Reference

1. Zumft, W.G., Paneque, A., Aparicio, P.J. and Losada, M. (1969). „Mechanism of nitrate reduction in Chlorella”. Biochem. Biophys. Res. Commun. 36: 980-986. PMID 4390523. 
2. Liang, J. and Burris, R.H. (1988). „Hydrogen burst associated with nitrogenase-catalyzed reactions”. Proc. Natl. Acad. Sci. USA 85: 9446-9450. PMID 3200830. 
3. Dance, I. (2004). „The mechanism of nitrogenase. Computed details of the site and geometry of binding of alkyne and alkene substrates and intermediates”. J. Am. Chem. Soc. 126: 11852-11863. PMID 15382920. 
4. Chan, J.M., Wu, W., Dean, D.R. and Seefeldt, L.C. (2000). „Construction and characterization of a heterodimeric iron protein: defining roles for adenosine triphosphate in nitrogenase catalysis”. Biochemistry 39: 7221-7228. PMID 10852721. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH Nitrogenase