L-cistein:1D-mio-inozitol 2-amino-2-dezoksi-alfa-D-glukopiranozidna ligaza

„L“-cistein:1D-mio-inozitol 2-amino-2-dezoksi-alfa-D-glukopiranozidna ligaza
Identifikatori
EC broj	6.3.1.13
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

L-cistein:1D-mio-inozitol 2-amino-2-dezoksi-alfa-D-glukopiranozidna ligaza (EC 6.3.1.13, MshC, MshC ligaza, Cys:GlcN-Ins ligaza, mikotiolna ligaza) je enzim sa sistematskim imenom L-cistein:1-O-(2-amino-2-dezoksi-alfa-D-glukopiranosil)-1D-mio-inozitol ligaza (formira AMP).^[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju

1-O-(2-amino-2-dezoksi-alfa-D-glukopiranozil)-1D-mio-inozitol + L-cistein + ATP ${\displaystyle \rightleftharpoons }$ 1-O-[2-(L-cisteinamido)-2-dezoksi-alfa-D-glukopiranozil]-1D-mio-inozitol + AMP + difosfat

Ovaj enzim je ključan za biosintezu mikotiola.

Reference

1. Fan, F., Luxenburger, A., Painter, G.F. and Blanchard, J.S. (2007). „Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC)”. Biochemistry 46: 11421-11429. PMID 17848100. 
2. Gutierrez-Lugo, M.T., Newton, G.L., Fahey, R.C. and Bewley, C.A. (2006). „Cloning, expression and rapid purification of active recombinant mycothiol ligase as B1 immunoglobulin binding domain of streptococcal protein G, glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis”. Protein Expr. Purif. 50: 128-136. PMID 16908186. 
3. Tremblay, L.W., Fan, F., Vetting, M.W. and Blanchard, J.S. (2008). „The 1.6 Å crystal structure of Mycobacterium smegmatis MshC: the penultimate enzyme in the mycothiol biosynthetic pathway”. Biochemistry 47: 13326-13335. PMID 19053270. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH L-cysteine:1D-myo-inositol+2-amino-2-deoxy-alpha-D-glucopyranoside+ligase