Inducibilni faktor hipoksije-asparagin dioksigenaza

Inducibilni faktor hipoksije-asparagin dioksigenaza
Identifikatori
EC broj	1.14.11.30
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Inducibilni faktor hipoksije-asparagin dioksigenaza (EC 1.14.11.30, HIF hidroksilaza) je enzim sa sistematskim imenom inducibilni faktor hipoksije-L-asparagin, 2-oksoglutarat:kiseonik oksidoreduktaza (4-hidroksilacija).^{[1][2][3][4][5][6]} Ovaj enzim katalizuje sledeću hemijsku reakciju

inducibilni faktor hipoksije-L-asparagin + 2-oksoglutarat + O₂ ${\displaystyle \rightleftharpoons }$ inducibilni faktor hipoksije-(3S)-3-hidroksi-L-asparagin + sukcinat + CO₂

Ovaj enzim sadrži gvožđe. Za njegov rad je neophodan askorbat. On katalizuje hidroksilaciju asparagina u C-terminalnom aktivacionom domenu HIF-alfa.

Reference

1. Mahon, P.C., Hirota, K. and Semenza, G.L. (2001). „FIH-1: a novel protein that interacts with HIF-1α and VHL to mediate repression of HIF-1 transcriptional activity”. Genes Dev. 15: 2675-2686. PMID 11641274. 
2. Hewitson, K.S., McNeill, L.A., Riordan, M.V., Tian, Y.M., Bullock, A.N., Welford, R.W., Elkins, J.M., Oldham, N.J., Bhattacharya, S., Gleadle, J.M., Ratcliffe, P.J., Pugh, C.W. and Schofield, C.J. (2002). „Hypoxia-inducible factor (HIF) asparagine hydroxylase is identical to factor inhibiting HIF (FIH) and is related to the cupin structural family”. J. Biol. Chem. 277: 26351-26355. PMID 12042299. 
3. Dann, C.E., 3rd, Bruick, R.K. and Deisenhofer, J. (2002). „Structure of factor-inhibiting hypoxia-inducible factor 1: An asparaginyl hydroxylase involved in the hypoxic response pathway”. Proc. Natl. Acad. Sci. USA 99: 15351-15356. PMID 12432100. 
4. Lando, D., Peet, D.J., Whelan, D.A., Gorman, J.J. and Whitelaw, M.L. (2002). „Asparagine hydroxylation of the HIF transactivation domain a hypoxic switch”. Science 295: 858-861. PMID 11823643. 
5. Koivunen, P., Hirsila, M., Gunzler, V., Kivirikko, K.I. and Myllyharju, J. (2004). „Catalytic properties of the asparaginyl hydroxylase (FIH) in the oxygen sensing pathway are distinct from those of its prolyl 4-hydroxylases”. J. Biol. Chem. 279: 9899-9904. PMID 14701857. 
6. Elkins, J.M., Hewitson, K.S., McNeill, L.A., Seibel, J.F., Schlemminger, I., Pugh, C.W., Ratcliffe, P.J. and Schofield, C.J. (2003). „Structure of factor-inhibiting hypoxia-inducible factor (HIF) reveals mechanism of oxidative modification of HIF-1 α”. J. Biol. Chem. 278: 1802-1806. PMID 12446723. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH Hypoxia-inducible+factor-asparagine+dioxygenase