HtrA2 peptidaza

HtrA2 peptidaza
Identifikatori
EC broj	3.4.21.108
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

HtrA2 peptidaza (EC 3.4.21.108, protein A2 sa zahtevom za visoku temperaturu, HtrA2, Omi stres-regulišuća endoproteaza, serinska proteinaza OMI, HtrA2 proteaza, OMI/HtrA2 proteaza, HtrA2/Omi, Omi/HtrA2) je enzim.^{[1][2][3][4][5]} Ovaj enzim katalizuje sledeću hemijsku reakciju

Odvajanje nepolarne alifatične aminokiseline na P1 poziciji, sa preferencijom za Val, Ile i Met. U P2 i P3 poziciji je najčešće Arg sa sekundarnom preferencijom za druge hidrofilne ostatake

Izražavanje ovog enzima je povišeno u sisarskim ćelijama u odgovoru na stres indukovan toplotnim šokom i tunikamicinskim tretmanom, a ima značaj i u patogenezi Parkinsonove bolesti.

Reference

1. Srinivasula, S.M., Gupta, S., Datta, P., Zhang, Z., Hegde, R., Cheong, N., Fernandes-Alnemri, T. and Alnemri, E.S. (2003). „Inhibitor of apoptosis proteins are substrates for the mitochondrial serine protease Omi/HtrA2”. J. Biol. Chem. 278: 31469-31472. PMID 12835328. 
2. Savopoulos, J.W., Carter, P.S., Turconi, S., Pettman, G.R., Karran, E.H., Gray, C.W., Ward, R.V., Jenkins, O. and Creasy, C.L. (2000). „Expression, purification, and functional analysis of the human serine protease HtrA2”. Protein. Expr. Purif. 19: 227-234. PMID 10873535. 
3. Martins, L.M., Turk, B.E., Cowling, V., Borg, A., Jarrell, E.T., Cantley, L.C. and Downward, J. (2003). „Binding specificity and regulation of the serine protease and PDZ domains of HtrA2/Omi”. J. Biol. Chem. 278: 49417-49427. PMID 14512424. 
4. Gray, C.W., Ward, R.V., Karran, E., Turconi, S., Rowles, A., Viglienghi, D., Southan, C., Barton, A., Fantom, K.G., West, A., Savopoulos, J., Hassan, N.J., Clinkenbeard, H., Hanning, C., Amegadzie, B., Davis, J.B., Dingwall, C., Livi, G.P. and Creasy, C.L. (2000). „Characterization of human HtrA2, a novel serine protease involved in the mammalian cellular stress response”. Eur. J. Biochem. 267: 5699-5710. PMID 10971580. 
5. Li, W., Srinivasula, S.M., Chai, J., Li, P., Wu, J.W., Zhang, Z., Alnemri, E.S. and Shi, Y. (2002). „Structural insights into the pro-apoptotic function of mitochondrial serine protease HtrA2/Omi”. Nat. Struct. Biol. 9: 436-441. PMID 11967569. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH HtrA2+peptidase