Heliks-zavoj-heliks

λ represor bakteriofaga lambda koristi heliks-zavoj-heliks (levo; zeleno) da se veže za DNK (desno; plavo i crveno).

U proteinima, heliks-zavoj-heliks (HTH) je glavni strukturni motiv sa sposobnošću vezivanja za DNK. On se sastoji od dva α heliksa spojena kratkim lancom aminokiselina. Prisutan je u mnogim proteinima koji regulišu ekspresiju gena. On se razlikuje od domena heliks-petlja-heliks.^[1]

Otkriće

Otkriće heliks-zavoj-heliks motiva je bilo bazirano na sličnostima između nekoliko gena koji koriraju transkripcione regulatorne proteine iz bakteriofaga lambda i Escherichia coli: Cro, CAP, i λ represor, za koje je najđeno da imaju zajedničku 20-25 aminokiselina dugu sekvencu koja omogućava DNK prepoznavanje.^[2][3][4][5]

Reference

1. Brennan RG, Matthews BW (1989). „The helix-turn-helix DNA binding motif.”. J Biol Chem 264 (4): 1903–6. PMID 2644244. 
2. Matthews BW, Ohlendorf DH, Anderson WF, Takeda Y (1982). „Structure of the DNA-binding region of lac repressor inferred from its homology with cro repressor.”. Proc Natl Acad Sci U S A 79 (5): 1428–32. PMC 345986. PMID 6951187. 
3. Anderson WF, Ohlendorf DH, Takeda Y, Matthews BW (1981). „Structure of the cro repressor from bacteriophage lambda and its interaction with DNA.”. Nature 290 (5809): 754–8. PMID 6452580. 
4. McKay DB, Steitz TA (1981). „Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed B-DNA.”. Nature 290 (5809): 744–9. PMID 6261152. 
5. Pabo CO, Lewis M (1982). „The operator-binding domain of lambda repressor: structure and DNA recognition.”. Nature 298 (5873): 443–7. PMID 7088190. 

Literatura

• Struhl K (1989). „Helix-turn-helix, zinc-finger, and leucine-zipper motifs for eukaryotic transcriptional regulatory proteins.”. Trends Biochem Sci 14 (4): 137–40. PMID 2499084. 
• Gajiwala KS, Burley SK (2000). „Winged helix proteins.”. Curr Opin Struct Biol 10 (1): 110–6. PMID 10679470. 
• Santos CL, Tavares F, Thioulouse J, Normand P (2009). „A phylogenomic analysis of bacterial helix-turn-helix transcription factors.”. FEMS Microbiol Rev 33 (2): 411–29. DOI:10.1111/j.1574-6976.2008.00154.x. PMID 19076237. 
• Hoskisson PA, Rigali S (2009). „Chapter 1: Variation in form and function the helix-turn-helix regulators of the GntR superfamily.”. Adv Appl Microbiol 69: 1–22. DOI:10.1016/S0065-2164(09)69001-8. PMID 19729089. 
• Brennan RG (1993). „The winged-helix DNA-binding motif: another helix-turn-helix takeoff.”. Cell 74 (5): 773–6. PMID 8374950. 
• Huffman JL, Brennan RG (2002). „Prokaryotic transcription regulators: more than just the helix-turn-helix motif.”. Curr Opin Struct Biol 12 (1): 98–106. PMID 11839496. 

Povezano

• Domen vezivanja DNK
• Sekundarna struktura proteina
• Cinkov prst

Vanjske veze

• Heliks-zavoj-heliks
• PDB 1LMB Arhivirano 2004-10-29 na Wayback Machine-u
• Cro/C1-type HTH domen

Heliks-zavoj-heliks domeni

Bakterijski regulatorni heliks-zavoj-heliks protein, lysR familija Identifikatori Simbol HTH_1 Pfam PF00126 Pfam klan CL0123 InterPro IPR000847 PROSITE PDOC00043 SCOP 1al3 Superfamilija 1al3 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled HTH DNA vezujući domen Identifikatori Simbol HTH_10 Pfam PF04967 Pfam klan CL0123 InterPro IPR007050 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Ribonukleazni R heliksni domen Identifikatori Simbol HTH_12 Pfam PF08461 Pfam klan CL0123 InterPro IPR013668 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled HTH DNA vezujući domen Identifikatori Simbol HTH_13 Pfam PF11972 Pfam klan CL0123 InterPro IPR021068 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks Identifikatori Simbol HTH_3 Pfam PF01381 Pfam klan CL0123 InterPro IPR001387 SCOP 1r69 Superfamilija 1r69 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Bakterijski regulatorni protein, arsR familija Identifikatori Simbol HTH_5 Pfam PF01022 Pfam klan CL0123 InterPro IPR001845 PROSITE PDOC00661 SCOP 1smt Superfamilija 1smt Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks domen, rpiR familija Identifikatori Simbol HTH_6 Pfam PF01418 Pfam klan CL0123 InterPro IPR000281 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks domen rezolvaze Identifikatori Simbol HTH_7 Pfam PF02796 Pfam klan CL0123 InterPro IPR006120 PROSITE PDOC00334 SCOP 1hcr Superfamilija 1hcr Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Bakterijski regulatorni protein, Fis familija Identifikatori Simbol HTH_8 Pfam PF02954 Pfam klan CL0123 InterPro IPR002197 SCOP 1f36 Superfamilija 1f36 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled RNA polimeraza III podjedinica RPC82 heliks-zavoj-heliks domen Identifikatori Simbol HTH_9 Pfam PF08221 Pfam klan CL0123 InterPro IPR013197 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Bakterijski regulatorni heliks-zavoj-heliks proteini, AraC familija Identifikatori Simbol HTH_AraC Pfam PF00165 Pfam klan CL0123 InterPro IPR000005 PROSITE PDOC00040 SCOP 1bl0 Superfamilija 1bl0 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled CodY heliks-zavoj-heliks domen Identifikatori Simbol HTH_CodY Pfam PF08222 Pfam klan CL0123 InterPro IPR013198 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled DeoR sličan heliks-zavoj-heliks domen Identifikatori Simbol HTH_DeoR Pfam PF08220 Pfam klan CL0123 InterPro IPR001034 PROSITE PDOC00696 SCOP 1lk5 Superfamilija 1lk5 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled IclR heliks-zavoj-heliks domen Identifikatori Simbol HTH_IclR Pfam PF09339 Pfam klan CL0123 InterPro IPR005471 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled M protein trans delujući pozitivni regulator (MGA) HTH domen Identifikatori Simbol HTH_Mga Pfam PF08280 Pfam klan CL0123 InterPro IPR013199 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks domen Identifikatori Simbol HTH_OrfB_IS605 Pfam PF12323 Pfam klan CL0123 InterPro IPR021027 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks Psq domen Identifikatori Simbol HTH_psq Pfam PF05225 Pfam klan CL0123 InterPro IPR007889 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Transposaza Identifikatori Simbol HTH_Tnp_1 Pfam PF01527 Pfam klan CL0123 InterPro IPR002514 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Transposaza Identifikatori Simbol HTH_Tnp_IS630 Pfam PF01710 Pfam klan CL0123 InterPro IPR002622 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Mi DNK vezujući domen Identifikatori Simbol HTH_Tnp_Mu_1 Pfam PF02316 Pfam klan CL0123 InterPro IPR003314 SCOP 1qpm Superfamilija 1qpm Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Mi DNK Vezujući, I gama poddomen Identifikatori Simbol HTH_Tnp_Mu_2 Pfam PF09039 Pfam klan CL0123 InterPro IPR015126 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Transposaza Identifikatori Simbol HTH_Tnp_Tc3_2 Pfam PF01498 Pfam klan CL0123 InterPro IPR002492 SCOP 1tc3 Superfamilija 1tc3 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Tc5 transposaza DNA vezujući domen Identifikatori Simbol HTH_Tnp_Tc5 Pfam PF03221 Pfam klan CL0123 InterPro IPR004906 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Regulator sporulacije, WhiA C terminusni domen Identifikatori Simbol HTH_WhiA Pfam PF02650 Pfam klan CL0123 InterPro IPR003802 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks domen alkil merkuri liaze Identifikatori Simbol HTH_15 Pfam PF12324 Pfam klan CL0123 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks domen Identifikatori Simbol HTH_16 Pfam PF12645 Pfam klan CL0123 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks domen Identifikatori Simbol HTH_17 Pfam PF12728 Pfam klan CL0123 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks domen Identifikatori Simbol HTH_18 Pfam PF12833 Pfam klan CL0123 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks domen Identifikatori Simbol HTH_19 Pfam PF12844 Pfam klan CL0123 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks domen Identifikatori Simbol HTH_20 Pfam PF12840 Pfam klan CL0123 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled InsA C-terminusni domen Identifikatori Simbol HTH_Tnp_IS1 Pfam PF12759 Pfam klan CL0123 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Heliks-zavoj-heliks domen transposaze IS66 Identifikatori Simbol HTH_Tnp_IS66 Pfam PF13005 Pfam klan CL0123 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled HTH domen Identifikatori Simbol HTH_11 Pfam PF08279 Pfam klan CL0123 InterPro IPR013196 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled Tc3 transposaza Identifikatori Simbol HTH_Tnp_Tc3_1 Pfam PF11427 Pfam klan CL0123 Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled