Glikoprotein 6-a-L-fukoziltransferaza

Glikoprotein 6-a-L-fukoziltransferaza
Identifikatori
EC broj	2.4.1.68
CAS broj	2605491
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Glikoprotein 6-a-L-fukoziltransferaza (EC 2.4.1.68, GDP-fukoza-glikoproteinska fukoziltransferaza, GDP-L-Fuc:N-acetil-beta-D-glukozaminidna alfa1->6 fukoziltransferaza, GDP-L-fukoza-glikoproteinska fukoziltransferaza, glikoproteinska fukoziltransferaza, guanozin difosfofukoza-glikoproteinska fukoziltransferaza, GDP-L-fukoza:glikoprotein (L-fukoza to asparagin-vezani N-acetilglukozamin of 4-NN-acetil-beta-D-glukozaminil-(1->2)-alfa-D-manozil-(1->3)-(N-acetil-beta-D-glukozaminil-(1->2)-alfa-D-manozil-(1->6))-beta-D-manozil-(1->4)-N-acetil-beta-D-glukozaminil-(1->4)-N-acetil-beta-D-glukozaminil}asparagin) 6-alfa-L-fukoziltransferaza, FucT) je enzim sa sistematskim imenom GDP-beta-L-fukoza:glikoprotein (L-fukoza za asparagin-vezani N-acetilglukozamin N⁴- {N-acetil-beta-D-glukozaminil-(1->2)-alfa-D-manozil-(1->3)-(N-acetil-beta-D-glukozaminil-(1->2)-alfa-D-manozil-(1->6))-beta-D-manozil-(1->4)-N-acetil-beta-D-glukozaminil-(1->4)-N-acetil-beta-D-glukozaminil}asparagin) 6-alfa-L-fukoziltransferaza.^[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju

GDP-beta-L-fukoza + N⁴-{N-acetil-beta-D-glukozaminil-(1->2)-alfa-D-manozil-(1->3)-[N-acetil-beta-D-glukozaminil-(1->2)-alfa-D-manozil-(1->6)]-beta-D-manozil-(1->4)-N-acetil-beta-D-glukozaminil-(1->4)-N-acetil-beta-D-glukozaminil}asparagin ${\displaystyle \rightleftharpoons }$ GDP + N⁴-{N-acetil-beta-D-glukozaminil-(1->2)-alfa-D-manozil-(1->3)-[N-acetil-beta-D-glukozaminil-(1->2)-alfa-D-manozil-(1->6)]-beta-D-manozil-(1->4)-N-acetil-beta-D-glukozaminil-(1->4)-[alfa-L-fukozil-(1->6)]-N-acetil-beta-D-glukozaminil}asparagin

Ovaj enzim katalizuje reakciju sličnu reakciji koju posreduje enzim EC 2.4.1.214, glikoprotein 3-alfa-L-fukoziltransferaza.

Reference

1. Longmore, G.D. and Schachter, H. (1982). „Product-identification and substrate-specificity studies of the GDP-L-fucose:2-acetamido-2-deoxy-β-D-glucoside (Fuc → Asn-linked GlcNAc) 6-α-L-fucosyltransferase in a Golgi-rich fraction from porcine liver”. Carbohydr. Res. 100: 365-392. PMID 7083256. 
2. Voynow, J.A., Scanlin, T.F. and Glick, M.C. (1988). „A quantitative method for GDP-L-Fuc:N-acetyl-β-D-glucosaminide α1→6fucosyltransferase activity with lectin affinity chromatography”. Anal. Biochem. 168: 367-373. PMID 3364733. 
3. Uozumi, N., Yanagidani, S., Miyoshi, E., Ihara, Y., Sakuma, T., Gao, C.-X., Teshima, T., Fujii, S., Shiba, T. and Taniguchi, N. (1996). „Purification and cDNA cloning of porcine brain GDP-L-Fuc:N-acetyl-β-D-glucosaminide α1→6fucosyltransferase”. J. Biol. Chem. 271: 27810-27817. PMID 8910378. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH Glycoprotein+6-alpha-L-fucosyltransferase