Glikolipid 2-a-manoziltransferaza

Glikolipid 2-a-manoziltransferaza
Identifikatori
EC broj	2.4.1.131
CAS broj	74506-43-7
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Glikolipid 2-a-manoziltransferaza (EC 2.4.1.131, ALG11, ALG11 manoziltransferaza, LEW3 (gen), At2G40190 (gen), gmd3 (gen), protein galaktomananske deficijencije 3, GDP-manoza:glikolipid 1,2-alfa-D-manoziltransferaza, glikolipid 2-alfa-manoziltransferaza, GDP-manoza:glikolipid 2-alfa-D-manoziltransferaza, GDP-Man:Man3GlcNAc2-PP-Dol alfa-1,2-manoziltransferaza) je enzim sa sistematskim imenom GDP-alfa-D-manoza:D-Man-alfa-(1->3)-(D-Man-alfa-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-difosfodolihol 2-alfa-D-manoziltransferaza.^[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju

2 GDP-alfa-D-manoza + D-MaN-alfa-(1->3)-[D-MaN-alfa-(1->6)]-D-MaN-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-difosfodolihol ${\displaystyle \rightleftharpoons }$ 2 GDP + D-MaN-alfa-(1->2)-D-MaN-alfa-(1->2)-D-MaN-alfa-(1->3)-[D-MaN-alfa-(1->6)]-D-MaN-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-difosfodolihol

Pri biosintezi za asparagin vezanih glikoproteina (N-vezanoj proteinskoj glikozilaciji) se koristi za dolihil difosfat vezani glikozilni donor. Njega formira serija za membranu vezanih glikoziltransferaza.

Reference

1. O'Reilly, M.K., Zhang, G. and Imperiali, B. (2006). „In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis”. Biochemistry 45: 9593-9603. PMID 16878994. 
2. Absmanner, B., Schmeiser, V., Kampf, M. and Lehle, L. (2010). „Biochemical characterization, membrane association and identification of amino acids essential for the function of Alg11 from Saccharomyces cerevisiae, an α1,2-mannosyltransferase catalysing two sequential glycosylation steps in the formation of the lipid-linked core oligosaccharide”. Biochem. J. 426: 205-217. PMID 19929855. 
3. Schutzbach, J.S., Springfield, J.D. and Jensen, J.W. (1980). „The biosynthesis of oligosaccharide-lipids. Formation of an α-1,2-mannosyl-mannose linkage”. J. Biol. Chem. 255: 4170-4175. PMID 6154707. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH GDP-Man:Man3GlcNAc2-PP-dolichol+alpha-1,2-mannosyltransferase