Aerobaktinska sintaza

Aerobaktinska sintaza
Identifikatori
EC broj	6.3.2.39
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Aerobaktinska sintaza (EC 6.3.2.39, iucC (gen)) je enzim sa sistematskim imenom N²-citril-N⁶-acetil-N⁶-hidroksi-L-lizin:N⁶-acetil-N⁶-hidroksi-L-lizin ligaza (formira ADP).^{[1][2][3][4][5][6]} Ovaj enzim katalizuje sledeću hemijsku reakciju

2 ATP + N²-citril-N⁶-acetil-N⁶-hidroksi-L-lizin + N⁶-acetil-N⁶-hidroksi-L-lizin + H₂O ${\displaystyle \rightleftharpoons }$ 2 ADP + 2 fosfat + aerobaktin

Za dejstvo ovog enzima je neophodan jon Mg²⁺.

Reference

1. Appanna, D.L., Grundy, B.J., Szczepan, E.W. and Viswanatha, T. (1984). „Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1”. Biochim. Biophys. Acta 801: 437-443. 
2. Gibson, F. and Magrath, D.I. (1969). „The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I”. Biochim. Biophys. Acta 192: 175-184. PMID 4313071. 
3. Maurer, P.J. and Miller, M. (1982). „Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N⁶-acetyl-N⁶-hydroxylysine”. J. Am. Chem. Soc. 104: 3096-3101. 
4. de Lorenzo, V., Bindereif, A., Paw, B.H. and Neilands, J.B. (1986). „Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12”. J. Bacteriol. 165: 570-578. PMID 2935523. 
5. de Lorenzo, V. and Neilands, J.B. (1986). „Characterization of iucA and iucC genes of the aerobactin system of plasmid ColV-K30 in Escherichia coli”. J. Bacteriol. 167: 350-355. PMID 3087960. 
6. Challis, G.L. (2005). „A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases”. ChemBioChem 6: 601-611. PMID 15719346. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH Aerobactin+synthase