Acireduktonska sintaza

Acireduktonska sintaza
Identifikatori
EC broj	3.1.3.77
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Acireduktonska sintaza (EC 3.1.3.77, E1, E-1 enolaza-fosfataza) je enzim sa sistematskim imenom 5-(metiltio)-2,3-dioksopentil-fosfat fosfohidrolaza (izomerizacija).^[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju

5-(metiltio)-2,3-dioksopentil fosfat + H₂O ${\displaystyle \rightleftharpoons }$ 1,2-dihidroksi-5-(metiltio)pent-1-en-3-on + fosfat (sveukupna reakcija)

(1a) 5-(metiltio)-2,3-dioksopentil fosfat ${\displaystyle \rightleftharpoons }$ 2-hidroksi-5-(metiltio)-3-oksopent-1-enil fosfat (verovatno spontana reakcija)

(1b) 2-hidroksi-5-(metiltio)-3-oksopent-1-enil fosfat + H₂O ${\displaystyle \rightleftharpoons }$ 1,2-dihidroksi-5-(metiltio)pent-1-en-3-on + fosfat

Ovaj bifunkcionalanni enzim prvo enolizuje supstrat i formira intermedijer 2-hidroksi-5-(metiltio)-3-oksopent-1-enil fosfat, koji se zatim defosforiliše do aciredukton 1,2-dihidroksi-5-(metiltio)pent-1-en-3-ona.

Reference

1. Myers, R.W., Wray, J.W., Fish, S. and Abeles, R.H. (1993). „Purification and characterization of an enzyme involved in oxidative carbon-carbon bond cleavage reactions in the methionine salvage pathway of Klebsiella pneumoniae”. J. Biol. Chem. 268: 24785-24791. PMID 8227039. 
2. Wray, J.W. and Abeles, R.H. (1995). „The methionine salvage pathway in Klebsiella pneumoniae and rat liver. Identification and characterization of two novel dioxygenases”. J. Biol. Chem. 270: 3147-3153. PMID 7852397. 
3. Wang, H., Pang, H., Bartlam, M. and Rao, Z. (2005). „Crystal structure of human E1 enzyme and its complex with a substrate analog reveals the mechanism of its phosphatase/enolase activity”. J. Mol. Biol. 348: 917-926. PMID 15843022. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH Acireductone+synthase