Abietadienol hidroksilaza

Abietadienol hidroksilaza
Identifikatori
EC broj	1.14.13.109
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

Abietadienol hidroksilaza (EC 1.14.13.109, CYP720B1, PtAO, abietadienolna hidroksilaza (nespecifična)) je enzim sa sistematskim imenom abieta-7,13-dien-18-ol,NADPH:kiseonik oksidoreduktaza (18-hidroksilacija).^[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju

abieta-7,13-dien-18-ol + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ abieta-7,13-dien-18-al + NADP⁺ + 2H₂O (sveukupna reakcija)

(1a) abieta-7,13-dien-18-ol + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ abieta-7,13-dien-18,18-diol + NADP⁺ + H₂O

(1b) abieta-7,13-dien-18,18-diol ${\displaystyle \rightleftharpoons }$ abieta-7,13-dien-18-al + H₂O (spontana reakcija)

Ovaj enzim je hem-tiolatni protein (P-450). On učestvuje u biosintezi abietinske kiseline.

Reference

1. Funk, C. and Croteau, R. (1994). „Diterpenoid resin acid biosynthesis in conifers: characterization of two cytochrome P450-dependent monooxygenases and an aldehyde dehydrogenase involved in abietic acid biosynthesis”. Arch. Biochem. Biophys. 308: 258-266. PMID 8311462. 
2. Funk, C., Lewinsohn, E., Vogel, B.S., Steele, C.L. and Croteau, R. (1994). „Regulation of oleoresinosis in grand fir (Abies grandis) (coordinate induction of monoterpene and diterpene cyclases and two cytochrome P450-dependent diterpenoid hydroxylases by stem wounding)”. Plant Physiol. 106: 999-1005. PMID 12232380. 
3. Ro, D.K., Arimura, G., Lau, S.Y., Piers, E. and Bohlmann, J. (2005). „Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multifunctional, multisubstrate cytochrome P450 monooxygenase”. Proc. Natl. Acad. Sci. USA 102: 8060-8065. PMID 15911762. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH Abietadienol+hydroxylase