(N-acetilneuraminil)-galaktozilglukozilkeramid N-acetilgalaktozaminiltransferaza

(N-acetilneuraminil)-galaktozilglukozilkeramid N-acetilgalaktozaminiltransferaza
Identifikatori
EC broj	2.4.1.92
CAS broj	67338-98-1
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

(N-acetilneuraminil)-galaktozilglukozilkeramid N-acetilgalaktozaminiltransferaza (EC 2.4.1.92, uridin difosfoacetilgalaktozamin-gangliozid GM3 acetilgalaktozaminiltransferaza, gangliozid GM2 sintaza, gangliozid GM3 acetilgalaktozaminiltransferaza, GM2 sintaza, UDP acetilgalaktozamin-(N-acetilneuraminil)-D-galaktozil-D-glukozilkeramid acetilgalaktozaminiltransferaza, UDP-N-acetil-D-galaktozamin:1-O-(O-(N-acetil-alfa-neuraminil)-(2->3)-O-beta-D-galaktopiranozil-(1->4)-beta-D-glukopiranozil)-keramid 1,4-beta-N-acetil-D-galaktozaminiltransferaza acetilgalaktozaminiltransferaza, UDP-N-acetilgalaktozamin GM3 N-acetilgalaktozaminiltransferaza, uridin difosfoacetilgalaktozamin-acetilneuraminilgalaktozilglukozilkeramid acetilgalaktozaminiltransferaza, uridin difosfoacetilgalaktozamin-hematozid acetilgalaktozaminiltransferaza, GM2/GD2-sintaza, beta-1,4N-aetilgalaktozaminiltransferaza, asialo-GM2 sintaza, GalNAc-T, UDP-N-acetil-D-galaktozamin:(N-acetilneuraminil)-D-galaktozil-D-glukozilkeramid N-acetil-D-galaktozaminiltransferaza) je enzim sa sistematskim imenom UDP-N-acetil-D-galaktozamin:1-O-(O-(N-acetil-alfa-neuraminil)-(2->3)-O-beta-D-galaktopiranosil-(1->4)-beta-D-glukopiranosil)-keramid 4-beta-N-acetil-D-galaktozaminiltransferaza.^{[1][2][3][4][5][6][7]} Ovaj enzim katalizuje sledeću hemijsku reakciju

UDP-N-acetil-D-galaktozamin + 1-O-[O-(N-acetil-alfa-neuraminil)-(2->3)-O-beta-D-galaktopiranozil-(1->4)-beta-D-glukopiranozil]-keramid ${\displaystyle \rightleftharpoons }$ UDP + 1-O-[O-2-(acetilamino)-2-dezoksi-beta-D-galaktopiranozil-(1->4)-O-[N-acetil-alfa-neuraminil-(2->3)]-O-beta-D-galaktopiranozil-(1->4)-beta-D-glukopiranozil]-keramid

Ovaj enzim katalizuje formiranje gangliozida.

Reference

1. Dicesare, J.L. and Dain, J.A. (1971). „The enzymic synthesis of ganglioside. IV. UDP-N-acetylgalactosamine: (N-acetylneuraminyl)-galactosylglucosyl ceramide N-acetylgalactosaminyltransferase in rat brain”. Biochim. Biophys. Acta 231: 385-393. PMID 5554906. 
2. Pohlentz, G., Klein, D., Schwarzmann, G., Schmitz, D. and Sandhoff, K. (1988). „Both GA2, GM2, and GD2 synthases and GM1b, GD1a, and GT1b synthases are single enzymes in Golgi vesicles from rat liver”. Proc. Natl. Acad. Sci. USA 85: 7044-7048. PMID 3140234. 
3. Kazuya, I.-P., Hidari, J.K., Ichikawa, S., Furukawa, K., Yamasaki, M. and Hirabayashi, Y. (1994). „β1-4N-Acetylgalactosaminyltransferase can synthesize both asialoglycosphingolipid GM2 and glycosphingolipid GM2 in vitro and in vivo: isolation and characterization of a β1-4N-acetylgalactosaminyltransferase cDNA clone from rat ascites hepatoma cell line AH7974F”. Biochem. J. 303: 957-965. PMID 7980468. 
4. Hashimoto, Y., Sekine, M., Iwasaki, K. and Suzuki, A. (1993). „Purification and characterization of UDP-N-acetylgalactosamine G_M3/G_D3 N-acetylgalactosaminyltransferase from mouse liver”. J. Biol. Chem. 268: 25857-25864. PMID 8245020. 
5. Nagai, K. and Ishizuka, I. (1987). „Biosynthesis of monosulfogangliotriaosylceramide and GM2 by N-acetylgalactosaminyltransferase from rat brain”. J. Biochem. (Tokyo) 101: 1115-1127. PMID 3115968. 
6. Furukawa, K., Takamiya, K. and Furukawa, K. (2002). „β1,4-N-Acetylgalactosaminyltransferase—GM2/GD2 synthase: a key enzyme to control the synthesis of brain-enriched complex gangliosides”. Biochim. Biophys. Acta 1573: 356-362. PMID 12417418. 
7. Yamashita, T., Wu, Y.P., Sandhoff, R., Werth, N., Mizukami, H., Ellis, J.M., Dupree, J.L., Geyer, R., Sandhoff, K. and Proia, R.L. (2005). „Interruption of ganglioside synthesis produces central nervous system degeneration and altered axon-glial interactions”. Proc. Natl. Acad. Sci. USA 102: 2725-2730. PMID 15710896. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH (N-acetylneuraminyl)-galactosylglucosylceramide+N-acetylgalactosaminyltransferase