EosFP

Abbozzo proteine
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EosFP
Diagramma a nastro della forma rossa di EosFP
Proteina
UniProtQ5S6Z9
PDB2BTJ
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La EosFP è una proteina tetramerica che ha la proprietà di emettere una fluorescenza di colore dal verde al rosso; è utilizzata come marker in biologia molecolare.[1]

Lobophyllia hemprichii

La EosFP fu isolata per la prima volta nella sclerattinia Lobophyllia hemprichii[1]. Successivamente è stata clonata in Escherichia coli.[1]

È composta da 226 aminoacidi e ha una massa molecolare di 25.8 kDa.

La proteina emette inizialmente ua fluorescenza verde (516 nm) che vira al rosso (581 nm) se sottoposta a irradiazione ultravioletta.[2]

Note

  1. ^ a b c (EN) Wiedenmann J. et al, EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion, vol. 101, n. 45, 2004, pp. 15905–15910, DOI:10.1073/pnas.0403668101.
  2. ^ (EN) Ivanchenko S., Röcker C., Oswald F., Wiedenmann J., Nienhaus G.U., Targeted Green-Red Photoconversion of EosFP, a Fluorescent Marker Protein, in Journal of Biological Physics, vol. 31, 3–4, 2005, pp. 249–259, DOI:10.1007/s10867-005-0174-z, ISSN 0092-0606 (WC · ACNP).

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