UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase

UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
Identifiers
EC no.	2.3.1.191
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (EC 2.3.1.191, UDP-3-O-acyl-glucosamine N-acyltransferase, UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase, acyltransferase LpxD, acyl-ACP:UDP-3-O-(3-hydroxyacyl)-GlcN N-acyltransferase, firA (gene), lpxD (gene)) is an enzyme with systematic name (3R)-3-hydroxymyristoyl-(acyl-carrier protein):UDP-3-O-((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine N-acetyltransferase.^{[1][2][3][4][5]} This enzyme catalyses the following chemical reaction

(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine ${\displaystyle \rightleftharpoons }$ UDP-2,3-bis[O-(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine + holo-[acyl-carrier protein]

The enzyme catalyses a step of lipid A biosynthesis.

References

1. Bartling CM, Raetz CR (September 2009). "Crystal structure and acyl chain selectivity of Escherichia coli LpxD, the N-acyltransferase of lipid A biosynthesis". Biochemistry. 48 (36): 8672–83. doi:10.1021/bi901025v. PMC 2748855. PMID 19655786.
2. Buetow L, Smith TK, Dawson A, Fyffe S, Hunter WN (March 2007). "Structure and reactivity of LpxD, the N-acyltransferase of lipid A biosynthesis". Proceedings of the National Academy of Sciences of the United States of America. 104 (11): 4321–6. Bibcode:2007PNAS..104.4321B. doi:10.1073/pnas.0606356104. PMC 1810333. PMID 17360522.
3. Bartling CM, Raetz CR (May 2008). "Steady-state kinetics and mechanism of LpxD, the N-acyltransferase of lipid A biosynthesis". Biochemistry. 47 (19): 5290–302. doi:10.1021/bi800240r. PMC 2435086. PMID 18422345.
4. Kelly TM, Stachula SA, Raetz CR, Anderson MS (September 1993). "The firA gene of Escherichia coli encodes UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. The third step of endotoxin biosynthesis". The Journal of Biological Chemistry. 268 (26): 19866–74. doi:10.1016/S0021-9258(19)36593-7. PMID 8366125.
5. Bainbridge BW, Karimi-Naser L, Reife R, Blethen F, Ernst RK, Darveau RP (July 2008). "Acyl chain specificity of the acyltransferases LpxA and LpxD and substrate availability contribute to lipid A fatty acid heterogeneity in Porphyromonas gingivalis". Journal of Bacteriology. 190 (13): 4549–58. doi:10.1128/jb.00234-08. PMC 2446808. PMID 18456814.

External links

• UDP-3-O-(3-hydroxymyristoyl)glucosamine+N-acyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)