S-Adenozilmetionin:tRNA ribosyltransferase-isomerase

S-Adenosylmethionine:tRNA ribosyltransferase-isomerase
Identifiers
EC no.	2.4.99.17
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

S-Adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 2.4.99.17, QueA enzyme, queuosine biosynthesis protein QueA) is an enzyme with systematic name S-adenosyl-L-methionine:7-aminomethyl-7-deazaguanosine ribosyltransferase (ribosyl isomerizing; L-methionine, adenine releasing).^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine³⁴ in tRNA ${\displaystyle \rightleftharpoons }$ L-methionine + adenine + epoxyqueuosine³⁴ in tRNA

The reaction is a combined transfer and isomerization of the ribose moiety of S-adenosyl-L-methionine to the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn.

References

1. Slany RK, Bösl M, Crain PF, Kersten H (August 1993). "A new function of S-adenosylmethionine: the ribosyl moiety of AdoMet is the precursor of the cyclopentenediol moiety of the tRNA wobble base queuine". Biochemistry. 32 (30): 7811–7. doi:10.1021/bi00081a028. PMID 8347586.
2. Slany RK, Bösl M, Kersten H (1994). "Transfer and isomerization of the ribose moiety of AdoMet during the biosynthesis of queuosine tRNAs, a new unique reaction catalyzed by the QueA protein from Escherichia coli". Biochimie. 76 (5): 389–93. doi:10.1016/0300-9084(94)90113-9. PMID 7849103.
3. Kinzie SD, Thern B, Iwata-Reuyl D (May 2000). "Mechanistic studies of the tRNA-modifying enzyme QueA: a chemical imperative for the use of AdoMet as a "ribosyl" donor". Organic Letters. 2 (9): 1307–10. doi:10.1021/ol005756h. PMID 10810734.
4. Van Lanen SG, Iwata-Reuyl D (May 2003). "Kinetic mechanism of the tRNA-modifying enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA)". Biochemistry. 42 (18): 5312–20. doi:10.1021/bi034197u. PMID 12731872.
5. Mathews I, Schwarzenbacher R, McMullan D, Abdubek P, Ambing E, Axelrod H, et al. (June 2005). "Crystal structure of S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA) from Thermotoga maritima at 2.0 Å resolution reveals a new fold". Proteins. 59 (4): 869–74. doi:10.1002/prot.20419. PMID 15822125.
6. Grimm C, Ficner R, Sgraja T, Haebel P, Klebe G, Reuter K (December 2006). "Crystal structure of Bacillus subtilis S-adenosylmethionine:tRNA ribosyltransferase-isomerase". Biochemical and Biophysical Research Communications. 351 (3): 695–701. doi:10.1016/j.bbrc.2006.10.096. PMID 17083917.

External links

• S-adenosylmethionine:tRNA+ribosyltransferase-isomerase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)