Phthalate 4,5-dioxygenase

phthalate 4,5-dioxygenase
Identifiers
EC no.	1.14.12.7
CAS no.	63626-44-8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a phthalate 4,5-dioxygenase (EC 1.14.12.7) is an enzyme that catalyzes the chemical reaction

phthalate + NADH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD⁺

The 4 substrates of this enzyme are phthalate, NADH, H⁺, and O₂, whereas its two products are cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate and NAD⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor. The systematic name of this enzyme class is phthalate,NADH:oxygen oxidoreductase (4,5-hydroxylating). Other names in common use include PDO, and phthalate dioxygenase. This enzyme participates in 2,4-dichlorobenzoate degradation. It has 3 cofactors: iron, FMN, and Iron-sulfur.

References

• Batie CJ, LaHaie E, Ballou DP (1987). "Purification and characterization of phthalate oxygenase and phthalate oxygenase reductase from Pseudomonas cepacia". J. Biol. Chem. 262 (4): 1510–8. PMID 3805038.