N-hydroxythioamide S-beta-glucosyltransferase

thiohydroximate beta-D-glucosyltransferase
Identifiers
EC no.	2.4.1.195
CAS no.	9068-14-8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a N-hydroxythioamide S-beta-glucosyltransferase (EC 2.4.1.195) is an enzyme that catalyzes the chemical reaction

UDP-glucose + N-hydroxy-2-phenylethanethioamide ${\displaystyle \rightleftharpoons }$ UDP + desulfoglucotropeolin

Thus, the two substrates of this enzyme are UDP-glucose and N-hydroxy-2-phenylethanethioamide, whereas its two products are UDP and desulfoglucotropeolin.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-glucose:N-hydroxy-2-phenylethanethioamide S-beta-D-glucosyltransferase. Other names in common use include desulfoglucosinolate-uridine diphosphate glucosyltransferase, uridine diphosphoglucose-thiohydroximate glucosyltransferase, thiohydroximate beta-D-glucosyltransferase, UDPG:thiohydroximate glucosyltransferase, thiohydroximate S-glucosyltransferase, thiohydroximate glucosyltransferase, and UDP-glucose:thiohydroximate S-beta-D-glucosyltransferase.

References

• Jain JC, Reed DW, GrootWassink JW, Underhill EW (1989). "A radioassay of enzymes catalyzing the glucosylation and sulfation steps of glucosinolate biosynthesis in Brassica species". Anal. Biochem. 178 (1): 137–40. doi:10.1016/0003-2697(89)90369-2. PMID 2524977.
• Reed DW, Davin L, Jain JC, Deluca V, Nelson L, Underhill EW (1993). "Purification and properties of UDP-glucose:thiohydroximate glucosyltransferase from Brassica napus L. seedlings". Arch. Biochem. Biophys. 305 (2): 526–32. doi:10.1006/abbi.1993.1456. PMID 8373190.
• Fahey JW, Zalcmann AT, Talalay P (2001). "The chemical diversity and distribution of glucosinolates and isothiocyanates among plants". Phytochemistry. 56 (1): 5–51. doi:10.1016/S0031-9422(00)00316-2. PMID 11198818.
• TF; Abel S (2004). "Arabidopsis glucosyltransferase UGT74B1 functions in glucosinolate biosynthesis and auxin homeostasis". Plant J. 40 (6): 893–908. CiteSeerX 10.1.1.538.667. doi:10.1111/j.1365-313X.2004.02261.x. PMID 15584955.