Gene orders

Gene order is the permutation of genome arrangement. A fair amount of research has been done trying to determine whether gene orders evolve according to a molecular clock (molecular clock hypothesis) or in jumps (punctuated equilibrium). By comparing gene orders in dissimilar organisms, scientists are able to develop a molecular phylogeny tree.^[1] When organisms have similar gene orders, meaning they have likely diverged recently, it is called synteny.

Some research on gene orders in animals' mitochondrial genomes reveal that the mutation rate of gene orders is not a constant in some degrees.^[2]

Methods for genome mapping, determining the gene order, include:^[3][4]

• Radiation hybrid mapping
• Genetic linkage mapping (recombinant frequency)
• Fluorescent in situ hybridization (FISH)
• Restriction mapping
• Sequence-tagged site content mapping
• Southern hybridization
• PCR
• Whole genome sequencing (increasingly used as the costs of sequencing decline)

All of these methods can lead to a gene sequence or a DNA sequence by which genes can be identified and compared.

References

1. Singh, Pradeep Kumar; Singh, Pankaj; Singh, Rajat Pratap; Singh, Ram Lakhan (2021), "From gene to genomics", Advances in Animal Genomics, Elsevier, pp. 13–32, doi:10.1016/b978-0-12-820595-2.00002-3, ISBN 978-0-12-820595-2, retrieved 2024-04-29
2. Grusea, Simona (September 2010). "Measures for the exceptionality of gene order in conserved genomic regions". Advances in Applied Mathematics. 45 (3): 359–372. doi:10.1016/j.aam.2010.02.002.
3. Saraswathy, Nachimuthu; Ramalingam, Ponnusamy (2011-01-01), Saraswathy, Nachimuthu; Ramalingam, Ponnusamy (eds.), "6 - Genome mapping", Concepts and Techniques in Genomics and Proteomics, Woodhead Publishing Series in Biomedicine, Woodhead Publishing, pp. 77–93, doi:10.1533/9781908818058.77, ISBN 978-1-907568-10-7, retrieved 2024-04-29
4. Bishop, Martin J. (1998-01-01), Bishop, Martin J. (ed.), "5 - Comparative Mapping in Humans and Vertebrates", Guide to Human Genome Computing (Second Edition), San Diego: Academic Press, pp. 89–111, ISBN 978-0-12-102051-4, retrieved 2024-04-29