DNA-formamidopyrimidine glycosylase

DNA-formamidopyrimidine glycosylase
Identifiers
EC no.	3.2.2.23
CAS no.	78783-53-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

DNA-formamidopyrimidine glycosylase (EC 3.2.2.23, Fapy-DNA glycosylase, deoxyribonucleate glycosidase, 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase, 2,6-diamino-4-hydroxy-5(N-methyl)formamidopyrimidine-DNA glycosylase, formamidopyrimidine-DNA glycosylase, DNA-formamidopyrimidine glycosidase, Fpg protein) is an enzyme with systematic name DNA glycohydrolase (2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimide releasing).^[1] FPG is a base excision repair enzyme which recognizes and removes a wide range of oxidized purines from correspondingly damaged DNA.^[2] It was discovered by Zimbabwean scientist Christopher J. Chetsanga in 1975.^[3]

This enzyme catalyses the following chemical reaction

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine

This enzyme participates in processes leading to recovery from mutagenesis and/or cell death by alkylating agents.

References

1. Boiteux S, O'Connor TR, Laval J (October 1987). "Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein". The EMBO Journal. 6 (10): 3177–83. PMC 553760. PMID 3319582.
2. Serre1; Pereira De Jésus, K; Boiteux, S; Zelwer, C; Castaing, B; et al. (2002). "Crystal structure of the Lactococcus lactis formamidopyrimidine-DNA glycosylase bound to an abasic site analogue-containing DNA". The EMBO Journal. 21 (12): 2854–2865. doi:10.1093/emboj/cdf304. PMC 126059. PMID 12065399.
3. Chetsanga C.J., Lindahl T. (1979). "Release of 7-methylguanine residues whose imidazole rings have been opened from damaged DNA by a DNA glycosylase from Escherichia coli". Nucleic Acids Res. 6 (11): 3673–84. doi:10.1093/nar/6.11.3673. PMC 327965. PMID 386277.

External links

• DNA-formamidopyrimidine+glycosylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)