Aminopyrimidine aminohydrolase

Aminopyrimidine aminohydrolase
Identifiers
EC no.	3.5.99.2
CAS no.	9024-80-0
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Aminopyrimidine aminohydrolase (EC 3.5.99.2, thiaminase, thiaminase II, tenA (gene)) is an enzyme with systematic name 4-amino-5-aminomethyl-2-methylpyrimidine aminohydrolase.^{[1][2][3][4][5][6][7]} This enzyme catalyses the following chemical reaction

(1) 4-amino-5-aminomethyl-2-methylpyrimidine + H₂O ${\displaystyle \rightleftharpoons }$ 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia

(2) thiamine + H₂O ${\displaystyle \rightleftharpoons }$ 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

This enzyme was previously known as thiaminase II.

References

1. Fujita A, Nose Y (July 1954). "The second type of bacterial thiaminase". The Journal of Vitaminology. 1 (1): 1–7. doi:10.5925/jnsv1954.1.1. PMID 13243520.
2. Ikehata H (1960). "Purification of thiaminase II". J. Gen. Appl. Microbiol. 6: 30–39. doi:10.2323/jgam.6.30.
3. Toms AV, Haas AL, Park JH, Begley TP, Ealick SE (February 2005). "Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II". Biochemistry. 44 (7): 2319–29. doi:10.1021/bi0478648. PMID 15709744.
4. Benach J, Edstrom WC, Lee I, Das K, Cooper B, Xiao R, Liu J, Rost B, Acton TB, Montelione GT, Hunt JF (May 2005). "The 2.35 A structure of the TenA homolog from Pyrococcus furiosus supports an enzymatic function in thiamine metabolism". Acta Crystallographica Section D. 61 (Pt 5): 589–98. doi:10.1107/S0907444905005147. PMID 15858269.
5. Jenkins AH, Schyns G, Potot S, Sun G, Begley TP (August 2007). "A new thiamin salvage pathway". Nature Chemical Biology. 3 (8): 492–7. doi:10.1038/nchembio.2007.13. PMID 17618314.
6. Jenkins AL, Zhang Y, Ealick SE, Begley TP (February 2008). "Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis". Bioorganic Chemistry. 36 (1): 29–32. doi:10.1016/j.bioorg.2007.10.005. PMC 2293645. PMID 18054064.
7. French JB, Begley TP, Ealick SE (September 2011). "Structure of trifunctional THI20 from yeast". Acta Crystallographica Section D. 67 (Pt 9): 784–91. doi:10.1107/S0907444911024814. PMC 3169313. PMID 21904031.

External links

• Aminopyrimidine+aminohydrolase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)