25-hydroxycholesterol 7α-hydroxylase

25-hydroxycholesterol 7alpha-hydroxylase
Identifiers
EC no.	1.14.13.100
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

25-hydroxycholesterol 7alpha-hydroxylase (EC 1.14.13.100, 25-hydroxycholesterol 7alpha-monooxygenase, CYP7B1, CYP7B1 oxysterol 7alpha-hydroxylase) is an enzyme with systematic name cholest-5-ene-3beta,25-diol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating).^[1][2][3][4] This enzyme catalyses the following chemical reaction

(1) cholest-5-ene-3beta,25-diol + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ cholest-5-ene-3beta,7alpha,25-triol + NADP⁺ + H₂O

(2) cholest-5-ene-3beta,27-diol + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ cholest-5-ene-3beta,7alpha,27-triol + NADP⁺ + H₂O

25-hydroxycholesterol 7α-hydroxylase is a heme-thiolate protein (P-450).

See also

• cholesterol;
• 25-hydroxycholesterol.

References

1. Toll A, Wikvall K, Sudjana-Sugiaman E, Kondo KH, Björkhem I (September 1994). "7 alpha hydroxylation of 25-hydroxycholesterol in liver microsomes. Evidence that the enzyme involved is different from cholesterol 7 alpha-hydroxylase". European Journal of Biochemistry. 224 (2): 309–16. doi:10.1111/j.1432-1033.1994.00309.x. PMID 7925343.
2. Li-Hawkins J, Lund EG, Bronson AD, Russell DW (June 2000). "Expression cloning of an oxysterol 7alpha-hydroxylase selective for 24-hydroxycholesterol". The Journal of Biological Chemistry. 275 (22): 16543–9. doi:10.1074/jbc.M001810200. PMID 10748047.
3. Ren S, Marques D, Redford K, Hylemon PB, Gil G, Vlahcevic ZR, Pandak WM (May 2003). "Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the rat". Metabolism. 52 (5): 636–42. doi:10.1053/meta.2003.50106. PMID 12759897.
4. Russell DW (2003). "The enzymes, regulation, and genetics of bile acid synthesis". Annual Review of Biochemistry. 72: 137–74. doi:10.1146/annurev.biochem.72.121801.161712. PMID 12543708.

External links

• 25-hydroxycholesterol+7alpha-hydroxylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)