LCMT2

LCMT2
Identifikatori
Aliasi	LCMT2
Vanjski ID-jevi	OMIM: 611246 MGI: 1353659 HomoloGene: 19642 GeneCards: LCMT2
Lokacija gena (čovjek) Hrom. Hromosom 15 (čovjek)[1] Bend 15q15.3 Početak 43,323,649 bp[1] Kraj 43,330,582 bp[1]
Lokacija gena (miš) Hrom. Hromosom 2 (miš)[2] Bend 2|2 E5 Početak 120,958,788 bp[2] Kraj 120,971,134 bp[2]
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • GO:0102674, GO:0102675 methyltransferase activity • aktivnost sa transferazom • GO:0001948, GO:0016582 vezivanje za proteine • S-adenosylmethionine-dependent methyltransferase activity • tRNA methyltransferase activity • protein C-terminal leucine carboxyl O-methyltransferase activity Ćelijska komponenta • citoplazma • citosol Biološki proces • tRNA modification • Metilacija • tRNA methylation • wybutosine biosynthetic process • tRNA processing • C-terminal protein methylation Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	9836	329504
Ensembl	ENSG00000168806	ENSMUSG00000074890
UniProt	O60294	Q8BYR1
RefSeq (mRNK)	NM_014793	NM_177846
RefSeq (bjelančevina)	NP_055608	NP_808514
Lokacija (UCSC)	Chr 15: 43.32 – 43.33 Mb	Chr 2: 120.96 – 120.97 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Leucin-karboksil metiltransferaza 2 je enzim koji je kod ljudi kodiran genom LCMT2.^[5][6][7]

Protein koji je kodiran ovim genom bez introna pripada superporodici metiltransferaza i djeluje kao regulator kontrolne tačke G(1)/S i G(2)/M. Pretpostavlja se da oksidativni stres izazvan dimom cigareta i transformirajući faktor rasta beta 1 mogu inhibirati ćelijsku proliferaciju, modulirajući ekspresiju ovog proteina.^[7]

Hibridnom analizom zračenja, Nagase et al. (1998) mapirali su gen LCMT2 na hromosom 4. Međutim, Hartz (2013) ga je mapirala je na hromosom 15, sekvenca q15.3, a na osnovu poravnanja sekvence LCMT2 (GenBank AF265443) sa genomskom sekvencom (GRCh37).^[8][9]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 686 aminokiselina, a molekulska težina 75.602 Da .^[10].

10		20		30		40		50
MGPRSRERRA		GAVQNTNDSS		ALSKRSLAAR		GYVQDPFAAL		LVPGAARRAP
LIHRGYYVRA		RAVRHCVRAF		LEQIGAPQAA		LRAQILSLGA		GFDSLYFRLK
TAGRLARAAV		WEVDFPDVAR		RKAERIGETP		ELCALTGPFE		RGEPASALCF
ESADYCILGL		DLRQLQRVEE		ALGAAGLDAA		SPTLLLAEAV		LTYLEPESAA
ALIAWAAQRF		PNALFVVYEQ		MRPQDAFGQF		MLQHFRQLNS		PLHGLERFPD
VEAQRRRFLQ		AGWTACGAVD		MNEFYHCFLP		AEERRRVENI		EPFDEFEEWH
LKCAHYFILA		ASRGDTLSHT		LVFPSSEAFP		RVNPASPSGV		FPASVVSSEG
QVPNLKRYGH		ASVFLSPDVI		LSAGGFGEQE		GRHCRVSQFH		LLSRDCDSEW
KGSQIGSCGT		GVQWDGRLYH		TMTRLSESRV		LVLGGRLSPV		SPALGVLQLH
FFKSEDNNTE		DLKVTITKAG		RKDDSTLCCW		RHSTTEVSCQ		NQEYLFVYGG
RSVVEPVLSD		WHFLHVGTMA		WVRIPVEGEV		PEARHSHSAC		TWQGGALIAG
GLGASEEPLN		SVLFLRPISC		GFLWESVDIQ		PPITPRYSHT		AHVLNGKLLL
VGGIWIHSSS		FPGVTVINLT		TGLSSEYQID		TTYVPWPLML		HNHTSILLPE
EQQLLLLGGG		GNCFSFGTYF		NPHTVTLDLS		SLSAGQ

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija

Sekvenciranjem klonova dobijenih iz biblioteke moždane cDNK Nagase et al. (1998) klonirali su LCMT2, koji su označili KIAA0547. Transkript sadrži nekoliko ponavljajućih elemenata u svom 3-baznom UTR-u, a izvedeni protein sadrži 686 aminokiselina. RT-PCR je detektirao ekspresiju LCMT2 samo u bubrezima. In vitro translatirani LCMT3 imao je prividnu molekulsku masu od 61 kD, pomoću SDS-PAGE.

Reference

1. GRCh38: Ensembl release 89: ENSG00000168806 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000074890 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Aug 1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (1): 31–9. doi:10.1093/dnares/5.1.31. PMID 9628581.
6. Noma A, Suzuki T (Dec 2006). "Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis". Nucleic Acids Symp Ser (Oxf). 50 (50): 65–6. doi:10.1093/nass/nrl032. PMID 17150819.
7. "Entrez Gene: LCMT2 leucine carboxyl methyltransferase 2".
8. Hartz, P. A. Personal Communication. Baltimore, Md. 6/3/2013.
9. Nagase, T., Ishikawa, K., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 31-39, 1998. PubMed: 9628581
10. "UniProt, O60294". Pristupljeno 15. 7. 2021.

Dopunska literatura

• De Baere I, Derua R, Janssens V, et al. (2000). "Purification of porcine brain protein phosphatase 2A leucine carboxyl methyltransferase and cloning of the human homologue". Biochemistry. 38 (50): 16539–47. doi:10.1021/bi991646a. PMID 10600115.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Marwick JA, Kirkham P, Gilmour PS, et al. (2003). "Cigarette smoke-induced oxidative stress and TGF-beta1 increase p21waf1/cip1 expression in alveolar epithelial cells". Ann. N. Y. Acad. Sci. 973: 278–83. doi:10.1111/j.1749-6632.2002.tb04649.x. PMID 12485877.
• Ng CC, Arakawa H, Fukuda S, et al. (2003). "p53RFP, a p53-inducible RING-finger protein, regulates the stability of p21WAF1". Oncogene. 22 (28): 4449–58. doi:10.1038/sj.onc.1206586. PMID 12853982.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Kawamata N, Inagaki N, Mizumura S, et al. (2005). "Methylation status analysis of cell cycle regulatory genes (p16INK4A, p15INK4B, p21Waf1/Cip1, p27Kip1 and p73) in natural killer cell disorders". Eur. J. Haematol. 74 (5): 424–9. doi:10.1111/j.1600-0609.2005.00417.x. PMID 15813917.
• Noma A, Kirino Y, Ikeuchi Y, Suzuki T (2006). "Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA". EMBO J. 25 (10): 2142–54. doi:10.1038/sj.emboj.7601105. PMC 1462984. PMID 16642040.
• Liu FY, Qi JP, Xu FL, Wu AP (2006). "Clinicopathological and immunohistochemical analysis of gastrointestinal stromal tumor". World J. Gastroenterol. 12 (26): 4161–5. PMID 16830365.
• Moon SK, Choi YH, Kim CH, Choi WS (2006). "p38MAPK mediates benzyl isothiocyanate-induced p21WAF1 expression in vascular smooth muscle cells via the regulation of Sp1". Biochem. Biophys. Res. Commun. 350 (3): 662–8. doi:10.1016/j.bbrc.2006.09.092. PMID 17026958.