In enzymology , a flavonol synthase (EC 1.14.11.23 ) is an enzyme that catalyzes the following chemical reaction :
dihydroflavonol + 2-oxoglutarate + O2
⇌ ⇌ -->
{\displaystyle \rightleftharpoons }
O2 + H2 O
The 3 substrates of this enzyme are dihydroflavonol , 2-oxoglutarate , and O2 , whereas its 4 products are flavonol , succinate , CO2 , and H2 O .
This enzyme belongs to the family of oxidoreductases , specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor. The systematic name of this enzyme class is dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase . This enzyme participates in flavonoid biosynthesis .
References
Wellmann F, Lukacin R, Moriguchi T, Britsch L, Schiltz E, Matern U (2002). "Functional expression and mutational analysis of flavonol synthase from Citrus unshiu" . Eur. J. Biochem . 269 (16): 4134– 42. doi :10.1046/j.1432-1033.2002.03108.x PMID 12180990 . Lukacin R, Wellmann F, Britsch L, Martens S, Matern U (2003). "Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase". Phytochemistry 62 (3): 287– 92. doi :10.1016/S0031-9422(02)00567-8 . PMID 12620339 . Martens S, Forkmann G, Britsch L, Wellmann F, Matern U, Lukacin R (2003). "Divergent evolution of flavonoid 2-oxoglutarate-dependent dioxygenases in parsley" . FEBS Lett . 544 (1– 3): 93– 8. doi :10.1016/S0014-5793(03)00479-4 PMID 12782296 . CJ; Nakajima, J; Welford, RW; Yamazaki, M; Saito, K; Schofield, CJ (2004). "Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase, and flavanone 3beta-hydroxylase" . J. Biol. Chem . 279 (2): 1206– 16. doi :10.1074/jbc.M309228200 PMID 14570878 .
Activity Regulation Classification Kinetics Types
