2-methylcitrate synthase

2-methylcitrate synthase
Identifiers
EC no.	2.3.3.5
CAS no.	57827-78-8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a 2-methylcitrate synthase (EC 2.3.3.5) is an enzyme that catalyzes the chemical reaction

propanoyl-CoA + H₂O + oxaloacetate ${\displaystyle \rightleftharpoons }$ (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA

The 3 substrates of this enzyme are propanoyl-CoA, H₂O, and oxaloacetate, whereas its two products are (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate and CoA.

This enzyme belongs to the family of transferases, specifically those acyltransferases that convert acyl groups into alkyl groups on transfer. The systematic name of this enzyme class is propanoyl-CoA:oxaloacetate C-propanoyltransferase (thioester-hydrolysing, 1-carboxyethyl-forming). Other names in common use include 2-methylcitrate oxaloacetate-lyase, MCS, methylcitrate synthase, and methylcitrate synthetase. This enzyme participates in propanoate metabolism.

References

• Uchiyama H; Tabuchi T (1976). "Properties of methylcitrate synthase from Candida lipolytica". Agric. Biol. Chem. 40 (7): 1411–1418. doi:10.1271/bbb1961.40.1411.
• Buckel W; Wendisch, VF; De Graaf, AA; Müller, U; Linder, MI; Linder, D; Buckel, W (1997). "Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria". Arch. Microbiol. 168 (5): 428–36. doi:10.1007/s002030050518. PMID 9325432.
• Horswill AR, Escalante-Semerena JC (1999). "Salmonella typhimurium LT2 catabolizes propionate via the 2-methylcitric acid cycle". J. Bacteriol. 181 (18): 5615–23. PMC 94080. PMID 10482501.
• Wadman SK; Mienie, LJ; Erasmus, E; De Wet, WJ; Ketting, D; Duran, M; Wadman, SK (1994). "Identification of the stereoisomeric configurations of methylcitric acid produced by si-citrate synthase and methylcitrate synthase using capillary gas chromatography-mass spectrometry". J. Inherit. Metab. Dis. 17 (6): 738–47. doi:10.1007/BF00712017. PMID 7707698.