Pfu DNA polimerase

DNA, materi genetik yang terdapat di dalam seluruh makhluk hidup.

Pfu DNA polimerase merupakan salah satu jenis enzim yang digunakan dalam reaksi PCR (polymerase chain reaction).[1] PCR ini banyak digunakan dalam berbagai penelitian dibidang molekuler untuk memperbanyak sekuen DNA.[1] Enzim Pfu DNA polimerase mampu memperpanjang rantai DNA dalam suatu siklus. Sumber utama enzim ini adalah Pyrococcus furiosus.[2] Keunggulan utamanya dibandingkan dengan enzim DNA polimerase lainnya ialah waktu paruhnya yang sangat lama dan adanya mekanisme perbaikan rantai DNA yang salah (proofreading).[1] Selain itu, enzim ini bersifat termostabil atau bersifat tahan suhu tinggi.[2]

Mekanisme proofreading-nya adalah 3'-5' eksonuklease.[2] Melalui mekanisme proofreading, suatu kesalahan penambahan basa DNA saat amplifikasi dapat diperbaiki. Aktivitas proofreading ini memiliki keakuratan yang sangat tinggi sehingga enzim ini sering kali digunakan dalam reaksi pemanjangan primer, kloning, dan amplifikasi rantai DNA ujung tumpul (blunt end).[3]

Referensi

  1. ^ a b c Poliana J, MacCabe AP. 2007. Industrial Enzymes; Structure, Function, and Applications. Dordrecht: Springer. Halaman: 388. ISBN 978-1-4020-5376-4
  2. ^ a b c Brown TA. 1998. Biology Lab Fax.London: Academic Press. Halaman 171.
  3. ^ Angers M, Cloutier JF, Castonguay A, Drouin R. 2001. Optimal conditions to use Pfu exo(-) DNA polymerase for highly efficient ligation-mediated polymerase chain reaction protocols. Nucleic Acids Res 29.

Read other articles:

Frontera entre Tanzania y la República Democrática del Congo

Frontera entre Tanzania y la República Democrática del Congo Mapa de Tanzania. República Democrática del Congo República Democrática del Congo Tanzania Tanzania Longitud total 459 km[editar datos en Wikidata] La frontera entre la República Democrática del Congo y Tanzania es el lindero internacional continuo de 459 kilómetros de longitud que separa a la República Democrática del Congo de Tanzania en África Oriental.[1]​ Descripción La frontera está ubic...

 

 

Hardie Davis

American politician from Georgia Hardie DavisMayor of Augusta-Richmond CountyIn officeJanuary 1, 2015 – December 31, 2022Preceded byDeke CopenhaverSucceeded byGarnett L. JohnsonMember of the Georgia State Senatefrom the 22nd districtIn officeFebruary 8, 2010 – 2014Preceded byEd TarverSucceeded byHarold V. Jones IIMember of the Georgia House of Representativesfrom the 122nd districtIn officeJanuary 8, 2007 – 2009Preceded byPete WarrenSucceeded byEar...

 

 

Starfish Foundation

Non-profit organization This article has multiple issues. Please help improve it or discuss these issues on the talk page. (Learn how and when to remove these template messages) This article is an orphan, as no other articles link to it. Please introduce links to this page from related articles; try the Find link tool for suggestions. (March 2017) The topic of this article may not meet Wikipedia's notability guidelines for companies and organizations. Please help to demonstrate the notability...

Studio Ghibli

Japanese animation studio Studio Ghibli, Inc.The logo for Studio GhibliHeadquarters in Koganei, TokyoNative name株式会社スタジオジブリRomanized nameKabushiki gaisha Sutajio JiburiTypeSubsidiary (Kabushiki gaisha)IndustryMotion picturesVideo gamesTV commercialsPredecessorTopcraftFoundedJune 15, 1985; 38 years ago (1985-06-15)in Tokyo, JapanFoundersHayao MiyazakiToshio SuzukiIsao TakahataYasuyoshi TokumaHeadquartersKajino-chō, Koganei, Tokyo, JapanArea servedWorldw...

 

 

Фридрих Конрад Бейльштайн

Фридрих Конрад Бейльштайннім. Friedrich Konrad Beilstein Народився 17 лютого 1838(1838-02-17)[1][2][…]Санкт-Петербург, Російська імперіяПомер 18 жовтня 1906(1906-10-18)[1][2][…] (68 років)Санкт-Петербург, Російська імперіяПоховання Волковський цвинтарdКраїна  Російська імперіяДіяльн...

 

 

Gertrudenkapelle (Rheinbrohl)

Gertrudenkapelle, Blick zum romanischen Chorturm Die Gertrudenkapelle ist eine römisch-katholische Kapelle in der Ortsgemeinde Rheinbrohl im rheinland-pfälzischen Landkreis Neuwied. Geschichte Die der heiligen Gertrud von Nivelles geweihte Kapelle entstand in der ersten Hälfte des 13. Jahrhunderts als Gotteshaus des benachbarten Gertrudenhofes, der heute das Rathaus von Rheinbrohl beherbergt. Der Gertrudenhof befand sich im Besitz des Gertrudenstifts zu Nivelles. Das Stift verkaufte den Ho...

Blackstones F.C.

Association football club in England Football clubBlackstonesFull nameBlackstones Football ClubNickname(s)The Stones or BlackosFounded1891GroundLincoln Road, StamfordCapacity1,000 (100 seated)[1]ChairmanGary PeaceManagerJon Harrison & Ryan WoodLeagueUnited Counties League Division One2022–23United Counties League Division One, 8th of 21WebsiteClub website Home colours Away colours Half Moon Inn, St Pauls Street, Stamford Blackstones FC Original headquartersBlackstones Works site...

 

 

زالجاس زوماجولوف

هذه المقالة تحتاج للمزيد من الوصلات للمقالات الأخرى للمساعدة في ترابط مقالات الموسوعة. فضلًا ساعد في تحسين هذه المقالة بإضافة وصلات إلى المقالات المتعلقة بها الموجودة في النص الحالي. (سبتمبر 2023) زالجاس زوماجولوف   معلومات شخصية الميلاد 29 أغسطس 1988 (35 سنة)  أكتوبي  مواط

 

 

Втомне руйнування

Руйнування шатуна з алюмінієвого сплаву. Темна зона: втомне руйнування. Світла зона: зона крихкого доламу Вто́мне руйнува́ння — руйнування матеріалу під дією повторно-перемінних (часто циклічних) навантажень[1]. Фізичні причини втомного руйнування матеріалів доси

2012 Illinois Fighting Illini football team

American college football season 2012 Illinois Fighting Illini footballConferenceBig Ten ConferenceDivisionLeaders DivisionRecord2–10 (0–8 Big Ten)Head coachTim Beckman (1st season)Co-offensive coordinatorBilly Gonzales (1st season)Co-offensive coordinatorChris Beatty (1st season)Defensive coordinatorTim Banks (1st season)Captains Graham Pocic Ashante Williams Home stadiumMemorial Stadium(Capacity: 60,670)Seasons← 20112013 → 2012 Big Ten Conf...

 

 

العلاقات البحرينية النمساوية

العلاقات البحرينية النمساوية البحرين النمسا   البحرين   النمسا تعديل مصدري - تعديل   العلاقات البحرينية النمساوية هي العلاقات الثنائية التي تجمع بين البحرين والنمسا.[1][2][3][4][5] مقارنة بين البلدين هذه مقارنة عامة ومرجعية للدولتين: وجه المقا...

 

 

Albert-Édouard Janssen

Belgian politician, banker and professor Albert-Édouard JanssenBorn1 April 1883Antwerp, BelgiumDied29 March 1966(1966-03-29) (aged 82)Valduc Castle, Hamme-Mille, BelgiumNationalityBelgianOccupation(s)politician, banker, professorKnown formonetary theory and banking and financial sciences Albert-Édouard Janssen was a Belgian politician, banker and professor born in Antwerp, Belgium on 1 April 1883 and died in Valduc Castle in Hamme-Mille, Belgium on 29 March 1966. Biography Albert ...

Kandahar massacre

2012 murders by a U.S. soldier in Afghanistan Kandahar massacreLocation of the massacre in the Kandahar Province in AfghanistanLocationPanjwai District, Kandahar Province, AfghanistanCoordinates31°31′37″N 65°29′56″E / 31.527°N 65.499°E / 31.527; 65.499Date11 March 2012; 11 years ago (2012-03-11) 03:00 AFT (UTC+04:30)Attack typeTriple home invasion, spree killing, mass murderWeaponsM4 carbine with M203 grenade launcher and an M9 pistol. Som...

 

 

Dinamic Motorsport

Auto racing team This article contains weasel words: vague phrasing that often accompanies biased or unverifiable information. Such statements should be clarified or removed. (March 2021) Dinamic MotorsportCurrent seriesPorsche SupercupPorsche Carrera Cup ItaliaGT World Challenge Europe Endurance CupGT World Challenge Europe Sprint Cup24H SeriesADAC GT MastersInternational GT OpenCurrent drivers Klaus Bachler Matteo Cairoli Côme Ledogar Mauro Calamia Marius Nakken Giorgio Roda Alessandro Gia...

 

 

Thunder Thighs

This article is about the Kimya Dawson album. For the vocal group, see Thunderthighs. 2011 studio album by Kimya DawsonThunder ThighsStudio album by Kimya DawsonReleasedOctober 18, 2011Recorded2011GenreAnti-folk, Indie rockLength60:12LabelGreat Crap FactoryKimya Dawson chronology Alphabutt(2008) Thunder Thighs(2011) Thunder Thighs is Kimya Dawson's seventh solo album, released by Great Crap Factory on October 18, 2011. It received generally favorable reviews from critics.[1] P...

Dexanabinol

Chemical compound DexanabinolClinical dataATC codenoneIdentifiers IUPAC name (6aS,10aS)-9-(Hydroxymethyl)-6,6-dimethyl-3-(2-methyloctan-2-yl)-6a,7,10,10a-tetrahydrobenzo[c]chromen-1-ol CAS Number112924-45-5 YPubChem CID107778DrugBankDB06444 YChemSpider96934 NUNIIR6VT8U5372ChEMBLChEMBL334533CompTox Dashboard (EPA)DTXSID40150235 ECHA InfoCard100.201.022 Chemical and physical dataFormulaC25H38O3Molar mass386.576 g·mol−13D model (JSmol)Interactive image SMILES Oc2cc(cc1OC([...

 

 

Ilok Castle

For the Odescalchi castle in the Province of Rome, see Castello Orsini-Odescalchi. Ilok Castle Ilok Castle (Croatian: Iločki dvorac or Gradina Ilok) or Odescalchi Castle overlooks the town of Ilok in eastern Croatia. It is built on a hill above the town centre, offering views on the Danube and the Pannonian Plain. The castle was originally built in the 15th century by Nicholas of Ilok, Croatian viceroy and the king of Bosnia. The Ottomans conquered Ilok in the 16th century. After the victory...

 

 

Merensky Reef

Layer of igneous rock in the Bushveld igneous complex, South Africa Mines of the Merensky Reef The Merensky Reef is a layer of igneous rock in the Bushveld Igneous Complex (BIC) in the North West, Limpopo, Gauteng and Mpumalanga provinces of South Africa which together with an underlying layer, the Upper Group 2 Reef (UG2), contains most of the world's known reserves of platinum group metals (PGMs) or platinum group elements (PGEs)—platinum, palladium, rhodium, ruthenium, iridium and osmium...

Gallagher and Shean

Vaudevillian musical comedy double act of the early 20th century Gallagher and SheanSheet music to Mister Gallagher and Mister SheanNationalityAmericanYears active1910–1914 and 1920-1925GenresVaudeville, ComedyNotable works and rolesSong entitled, Absolutely, Mr. Gallagher? Positively, Mr. Shean!Former membersEd GallagherAl Shean Gallagher & Shean was a highly successful musical comedy double act in vaudeville and on Broadway in the 1910s and 1920s, consisting of Ed Gallagher (1873–19...

 

 

Championnats du monde de karaté 2023

Cet article est une ébauche concernant le karaté et la Hongrie. Vous pouvez partager vos connaissances en l’améliorant (comment ?) selon les recommandations des projets correspondants. Championnats du monde de karaté 2023 Généralités Sport Karaté Organisateur(s) Fédération mondiale de karaté Édition 26e Lieu(x) Budapest Date du 24 au 29 octobre 2023 Nations 103 Participants 1051 Navigation Édition précédente Édition suivante modifier Les Championnats du monde de karaté...

 

 

Strategi Solo vs Squad di Free Fire: Cara Menang Mudah!