Gelan tetrasaharidna nezasićeno glukuronilna hidrolaza

Gelan tetrasaharidna nezasićeno glukuronilna hidrolaza
Identifikatori
EC broj	3.2.1.179
Baze podataka
IntEnz	IntEnz pregled
BRENDA	BRENDA pristup
ExPASy	NiceZyme pregled
KEGG	KEGG pristup
MetaCyc	metabolički put
PRIAM	profil
Strukture PBP	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC članci PubMed članci NCBI proteini

Gelan tetrasaharidna nezasićeno glukuronilna hidrolaza (EC 3.2.1.179, UGL, hidrolaza nezasićenog glukuronila) je enzim sa sistematskim imenom beta-D-4-dezoksi-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alfa-L-Rhap-(1->3)-beta-D-Glcp beta-D-4-dezoksi-Delta4-GlcAp hidrolaza.^[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju

beta-D-4-dezoksi-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alfa-L-Rhap-(1->3)-beta-D-Glcp + H ₂O ${\displaystyle \rightleftharpoons }$

5-dehidro-4-dezoksi-D-glukuronat + beta-D-Glcp-(1->4)-alfa-L-Rhap-(1->3)-beta-D-Glcp

Ovaj enzim odvaja 4-dezoksi-4(5)-nezasićene D-glucuronske kiselina sa oligosaharida koje formiraju polisaharidne lijaze.

Reference

1. Itoh, T., Akao, S., Hashimoto, W., Mikami, B. and Murata, K. (2004). „Crystal structure of unsaturated glucuronyl hydrolase, responsible for the degradation of glycosaminoglycan, from Bacillus sp. GL1 at 1.8 Å resolution”. J. Biol. Chem. 279: 31804—31812. PMID 15148314. CS1 одржавање: Вишеструка имена: списак аутора (веза)
2. Hashimoto, W., Kobayashi, E., Nankai, H., Sato, N., Miya, T., Kawai, S. and Murata, K. (1999). „Unsaturated glucuronyl hydrolase of Bacillus sp. GL1: novel enzyme prerequisite for metabolism of unsaturated oligosaccharides produced by polysaccharide lyases”. Arch. Biochem. Biophys. 368: 367—374. PMID 10441389. CS1 одржавање: Вишеструка имена: списак аутора (веза)
3. Itoh, T., Hashimoto, W., Mikami, B. and Murata, K. (2006). „Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1”. Biochem. Biophys. Res. Commun. 344: 253—262. PMID 16630576. CS1 одржавање: Вишеструка имена: списак аутора (веза)

Literatura

• Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036. 
• Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097. 
• William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605. 

Spoljašnje veze

• Gellan+tetrasaccharide+unsaturated+glucuronyl+hydrolase на US National Library of Medicine Medical Subject Headings (MeSH)